Comparison of immunohistochemistry with PCR for assessment of ER, PR, and Ki-67 and prediction of pathological complete response in breast cancer

详细信息    查看全文

• 作者：Hans-Peter Sinn ; Andreas Schneeweiss ; Marius Keller ; Kornelia Schlombs…
• 关键词：Image analysis ; Breast cancer ; Ki67 ; mRNA ; RT ; qPCR ; Prediction ; Pathologic complete response ; neoadjuvant ; Immunohistochemistry (IHC) ; MammaTyper®
• 刊名：BMC Cancer
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：17
• 期：1
• 全文大小：1046KB
• 刊物主题：Cancer Research; Oncology; Surgical Oncology; Health Promotion and Disease Prevention; Biomedicine, general; Medicine/Public Health, general;
• 出版者：BioMed Central
• ISSN：1471-2407
• 卷排序：17

文摘

BackgroundProliferation may predict response to neoadjuvant therapy of breast cancer and is commonly assessed by manual scoring of slides stained by immunohistochemistry (IHC) for Ki-67 similar to ER and PgR. This method carries significant intra- and inter-observer variability. Automatic scoring of Ki-67 with digital image analysis (qIHC) or assessment of MKI67 gene expression with RT-qPCR may improve diagnostic accuracy.