Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles

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• 作者：Amrita Rai (2)
  Heike N?the (3)
  Nikolay Tzvetkov (2)
  Elena Korenbaum (4)
  Dietmar J. Manstein (1234) manstein.dietmar@mh-hannover.de
• 关键词：Dictyostelium discoideum – Dynamin – Presequence – Peroxisome – Fission – Cell adhesion
• 刊名：Cellular and Molecular Life Sciences (CMLS)
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：68
• 期：16
• 页码：2751-2767
• 全文大小：1.2 MB
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• 作者单位：1. Hannover Medical School, 30625 Hannover, Germany2. Institut für Biophysikalische Chemie, OE 4350, Medizinische Hochschule Hannover, Carl-Neuberg-Stra?e 1, 30623 Hannover, Germany3. Max-Planck-Institut für Medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany4. Forschungseinrichtung für Strukturanalyse, OE 8830, Medizinische Hochschule Hannover, Carl-Neuberg-Stra?e 1, 30623 Hannover, Germany
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Life Sciences
  Biochemistry
  
• 出版者：Birkh盲user Basel
• ISSN：1420-9071

文摘

Dictyostelium discoideum cells produce five dynamin family proteins. Here, we show that dynamin B is the only member of this group of proteins that is initially produced as a preprotein and requires processing by mitochondrial proteases for formation of the mature protein. Our results show that dynamin B-depletion affects many aspects of cell motility, cell-cell and cell-surface adhesion, resistance to osmotic shock, and fatty acid metabolism. The mature form of dynamin B mediates a wide range and unique combination of functions. Dynamin B affects events at the plasma membrane, peroxisomes, the contractile vacuole system, components of the actin-based cytoskeleton, and cell adhesion sites. The modulating effect of dynamin B on the activity of the contractile vacuole system is unique for the Dictyostelium system. Other functions displayed by dynamin B are commonly associated with either classical dynamins or dynamin-related proteins.