Diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on Listeria monocytogenes and Salmonella enterica serovar enteritidis in a co-culture model
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- 作者：Solomon H. Mariam ; Nigus Zegeye ; Abraham Aseffa ; Rawleigh Howe
- 关键词：Lactic acid bacteria ; L. monocytogenes ; S. Enteritidis ; Co ; culture ; Inhibition ; Viable but nonculturable
- 刊名：BMC Microbiology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：1351KB
- 刊物主题：Microbiology; Biological Microscopy; Mycology; Parasitology; Virology; Life Sciences, general;
- 出版者：BioMed Central
- ISSN：1471-2180
- 卷排序：17
文摘
BackgroundFood-borne infections cause huge economic and human life losses. Listeria monocytogenes and Salmonella enterica serovar Enteritidis are among the top ranking pathogens causing such losses. Control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to detect all such pathogens by culture-based methods. Modern methods such as flow cytometry allow analyses of cells at the single cell level within a short time and enable better and faster detection of such pathogens and distinctions between live and dead cells. Such methods should be complemented by control strategies including the use of beneficial bacteria that produce metabolites capable of inhibiting food-borne pathogens. In this study, broth cultures of lactic acid bacteria (LAB) isolated from fermented milk were tested for production of substances capable of inhibiting L. monocytogenes and S. Enteritidis in co-culture with LAB by assessment of colony-forming units (CFU) and live:dead cell populations by flow cytometry.