Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves

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• 作者：Adriana Pružinská ; Takayuki Shindo ; Sherry Niessen ; Farnusch Kaschani…
• 关键词：Senescence ; Activity ; based protein profiling ; Papain ; like proteases ; Vacuolar processing enzymes
• 刊名：BMC Plant Biology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：17
• 期：1
• 全文大小：2906KB
• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229
• 卷排序：17

文摘

BackgroundPapain-like Cys Proteases (PLCPs) and Vacuolar Processing Enzymes (VPEs) are amongst the most highly expressed proteases during leaf senescence in Arabidopsis. Using activity-based protein profiling (ABPP), a method that enables detection of active enzymes within a complex sample using chemical probes, the activities of PLCPs and VPEs were investigated in individually darkened leaves of Arabidopsis, and their role in senescence was tested in null mutants.