The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)

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• 作者：Li Su ; Zhengnan Li ; Mike Bernardy ; Paul A. Wiersma ; Zhihui Cheng…
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：160
• 期：10
• 页码：2651-2654
• 全文大小：1,925 KB
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• 作者单位：Li Su (1) (2)
  Zhengnan Li (2) (3)
  Mike Bernardy (2)
  Paul A. Wiersma (2)
  Zhihui Cheng (1)
  Yu Xiang (2)
  
  1. College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China
  2. Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, V0H 1Z0, Canada
  3. College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Pea streak virus (PeSV) is a member of the genus Carlavirus in the family Betaflexiviridae. Here, the first complete genome sequence of PeSV was determined by deep sequencing of a cDNA library constructed from dsRNA extracted from a PeSV-infected sample and Rapid Amplification of cDNA Ends (RACE) PCR. The PeSV genome consists of 8041 nucleotides excluding the poly(A) tail and contains six open reading frames (ORFs). The putative peptide encoded by the PeSV ORF6 has an estimated molecular mass of 6.6 kDa and shows no similarity to any known proteins. This differs from typical carlaviruses, whose ORF6 encodes a 12- to 18-kDa cysteine-rich nucleic-acid-binding protein. Electronic supplementary materialThe online version of this article (doi:10.-007/?s00705-015-2467-2) contains supplementary material, which is available to authorized users.A comment to this article is available at http://?dx.?doi.?org/-0.-007/?s00705-015-2594-9.