Analysis of β-galactosidase production and their genes of two strains of Lactobacillus bulgaricus
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- 作者：Wen Zhang (1) wendyzhang1982@163.com
Chuan Wang (2) wangchuan1974@sina.com
Cheng-Yu Huang (1) hcynuph@163.com
Qian Yu (2) yuqian560@163.com
Heng-Chuan Liu (2) lhc54@sohu.com
Chao-Wu Zhang (2) zhangcw42@sina.com
Xiao-Fang Pei (2) xxpei@sina.com
Xin Xu (2) xinxu62@163.com
Guo-Qing Wang (2) huaxiwgq@163.com - 关键词：β ; Galactosidase – β ; Galactosidase gene – Lactobacillus bulgaricus – Lactose intolerance
- 刊名：Biotechnology Letters
- 出版年：2012
- 出版时间：June 2012
- 年：2012
- 卷：34
- 期：6
- 页码：1067-1071
- 全文大小：327.5 KB
- 参考文献：1. Astapovich NI, Riabaia NE (2006) Patterns of growth and beta-galactosidase production by bifidobacteria. Mikrobiologiia 75:329–333
2. Corthesy B, Boris S, Lsler P et al (2005) Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects againse challenge with Helicobacter felis. J Infect Dis 192:1441–1449
3. Grano V, Diano N, Rossi S et al (2004) Production of low-lactose milk by means of nonisothermal bioreactors. Biotechnol Prog 20:1393–1401
4. Guo XH (2002) Fundamentals and applications of probiotics. Beijing Science and Technology Press, Beijing
5. Heyman MB, Committee on Nutrition (2006) Lactose intolerance in infants, children, and adolescents. Pediatrics 118:1279–1286
6. Hsu CA, Yu RC, Lee SL et al (2007) Cultural condition affecting the growth and production of beta-galactosidase by Bifidobacterium longum CCRC 15708 in a jar fermenter. Int J Food Microbiol 116:186–189
7. Li W, Zhang XM, Lu Y et al (2004) Lactose intolerance and the development of low-lactose milk. China Dairy 2:35–37
8. Masood MI, Qadir MI, Shirazi JH et al (2010) Beneficial effects of lactic acid bacteria on human beings. Crit Rev Microbiol 37:91–98
9. Novalin S, Neuhaus W, Kulbe KD (2005) A new innovative process to produce lactose-reduced skim milk. J Biotechnol 119:212–218
10. Panesar R, Panesar PS, Singh RS et al (2007) Applicability of alginate entrapped yeast cells for the production of lactose-hydrolyzed milk. J Food Process Eng 30:472–484
11. Pinheiro R, Belo I, Mota M (2003) Growth and beta-galactosidase activity in cultures of Kluyveromyces marxianus under increased air pressure. Lett Appl Microbiol 37:438–442
12. Wang C, Zhang CW, Pei XF et al (2007) Identification and phylogenetic analysis of one strain of Lactobacillus delbrueckii subsp. bulgaricus separated from yoghourt. Wei Shen Yan Jiu 36:713–718
13. Worrall DM, Goss NH (1989) The formation of biologically active beta-galactosidase inclusion bodies in Escherichia coli. Aust J Biotechnol 3:28–32
14. Zhang W, Wang C, Huang C et al (2011) Construction and expression of food-grade β-galactosidase gene in Lactococcus lactis. Curr Microbiol 62:639–644
15. Zhong Y (2003) Probiotic and the study of the lactose intolerance. For Med Sci Hyg 2:101–105 - 作者单位：1. Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, #16, Section?3, Renmin Nan Road, Chengdu, 610041 Sichuan, China2. Department of Medical Technology, West China School of Public Health, Sichuan University, #16, Section?3, Renmin Nan Road, Chengdu, 610041 Sichuan, China
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Biotechnology
Applied Microbiology
Biochemistry - 出版者：Springer Netherlands
- ISSN：1573-6776
文摘
A bacterial β-galactosidase delivery system is a potential therapy for lactose intolerance. Currently, two Lactobacillus bulgaricus strains with different biological characteristics are under consideration as potential sources. However, differences in these β-galactosidase genes and their resulting production levels are poorly characterized. The β-galactosidase ORF of L. bulgaricus yogurt isolate had high variability and was terminated at site 1924 due to a stop codon. However, the full 114 kDa β-galactosidase band was still resolved by SDS-PAGE, which may indicate that the interrupted ORF was translated into more than one peptide, and they together were folded into the complete enzyme protein that showed much higher β-galactosidase activity (6.2 U/mg protein) than the enzyme generated from L. bulgaricus reference strain (2.5 U/mg protein).