Isolation and Analysis of Salt Response of Lactobacillus plantarum FS5-5 from Dajiang

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• 作者：Xuefei Song ; Qianqian Wang ; Xin Xu ; Jingwei Lin…
• 关键词：Dajiang ; Lactobacillusplantarum FS5 ; 5 ; NaCl ; Protein
• 刊名：Indian Journal of Microbiology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：56
• 期：4
• 页码：451-460
• 全文大小：1,005 KB
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Springer India
• ISSN：0973-7715
• 卷排序：56

文摘

From 15 samples of dajiang collected in northeast of China, three salt resistant lactic acid bacteria were isolated and identified as Lactobacillusplantarum through physiological studies and 16S rDNA sequence alignment. L. plantarum FS5-5 showed better growth in an environment with 12 % (w/v) NaCl than the other two strains. The expression of proteins extracted from L.plantarum FS5-5 cultured in de Man, Rogosa, and Sharp (MRS) containing 0, 3, 6 and 9 % (w/v) NaCl was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS). The results showed that 42 kinds of proteins were identified, which could be divided into three groups: 27 kinds of proteins related to protein synthesis and degradation, six kinds of proteins related to carbohydrate metabolism and energy metabolism, nine proteins related to nucleic acid metabolism. Overexpression of these proteins imply that a series of changes have occurred in the process of protein synthesis and degradation, carbohydrate metabolism, energy metabolism and nucleic acid metabolism after L.plantarum FS5-5 exposed to salt stress. All these proteins may have effects on the salt-tolerant characteristics of the L.plantarum FS5-5.