Identification and genetic mapping of a recessive gene for resistance to stripe rust in wheat line LM168-1

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• 作者：Junyan Feng (1)
  Guoyue Chen (1)
  Yuming Wei (1)
  Yaxi Liu (2)
  Qiantao Jiang (1)
  Wei Li (3)
  Zhien Pu (3)
  Xiujin Lan (1)
  Shoufen Dai (1)
  Youliang Zheng (1) (2)
  
• 关键词：Wheat resistance gene ; Stripe rust ; Simple sequence repeat (SSR) ; Resistance gene analog polymorphism (RGAP) ; Target region amplification polymorphism (TRAP)
• 刊名：Molecular Breeding
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：33
• 期：3
• 页码：601-609
• 全文大小：262 KB
• 作者单位：Junyan Feng (1)
  Guoyue Chen (1)
  Yuming Wei (1)
  Yaxi Liu (2)
  Qiantao Jiang (1)
  Wei Li (3)
  Zhien Pu (3)
  Xiujin Lan (1)
  Shoufen Dai (1)
  Youliang Zheng (1) (2)
  
  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China
  2. Key Laboratory of Crop Germplasm Resources Utilization in Southwest China, Ministry of Agriculture, Sichuan Agricultural University, Ya’an, 625014, Sichuan, People’s Republic of China
  3. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China
  
• ISSN：1572-9788

文摘

Stripe rust (or yellow rust), caused by the fungus Puccinia striiformis f. sp. tritici (Pst), is one of the most important foliar diseases of wheat. Characterization and utilization of novel resistant genes is the most effective, economic and environmentally friendly approach to controlling the disease. Wheat line LM168-1, which was derived from a cross between common wheat Chuannong 16 and Milan, has good adult-plant resistance to stripe rust, based on field tests over several years. To elucidate the genetic basis of resistance, LM168-1 was crossed with susceptible variety SY95-71. Parents and F1, F2, BC1 and F2:3 progenies were tested in 2009-011 in a field inoculated with the predominant races of Pst in China. The genetic analysis showed that resistance to stripe rust in LM168-1 was controlled by a single recessive gene, temporarily designated yrLM168. Simple sequence repeat (SSR), resistance gene analog polymorphism (RGAP) and target region amplification polymorphism (TRAP) techniques were used to identify molecular markers linked to the resistance locus. Finally, a linkage group consisting of two SSR, four RGAP and five TRAP markers was constructed for yrLM168 with 102 F2 plants. The closest markers R1 and R2 flanked the resistance gene locus at 2.4 and 2.4?cM, respectively. Furthermore, two SSR markers Xwmc59 and Xwmc145 assigned the gene to chromosome 6A. Because yrLM168 confers high-level resistance to the predominant races of Pst in China, it should be useful in stripe rust resistance breeding programs. The closely linked markers can be used for rapidly transferring yrLM168 to wheat breeding populations.