Distribution of branched glycerol dialkyl glycerol tetraethers in soils on the Northeastern Qinghai-Tibetan Plateau and possible production by nitrite-reducing bacteria

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• 作者：ChiJun Sun ; ChuanLun Zhang ; FuYan Li ; HuanYe Wang…
• 关键词：bGDGTs ; Soil water content ; CBT ; nirS ; or nirK ; encoding bacteria ; Real time PCR ; Cluster analysis
• 刊名：Science China Earth Sciences
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：59
• 期：9
• 页码：1834-1846
• 全文大小：1,294 KB
• 刊物主题：Earth Sciences, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-1897
• 卷排序：59

文摘

Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are ubiquitous and abundant in soils, but their sources remain elusive. Recent studies demonstrate that the distributions of bGDGTs are sensitive to various environmental factors. In an effort to understand how and to what extent soil moisture (expressed as soil water content (SWC) or mean annual precipitation (MAP), pH and temperature may impact the distribution of bGDGTs, and to shed more light on the biological sources of bGDGTs in cold and arid regions, we investigated the distribution of bGDGTs as well as bacterial 16S rRNA gene and functional genes involved in the N cycle (including amoA, nirS and nirK) in 41 surface soil samples from around Lake Qinghai and east of Qaidam Basin on the Northeastern Qinghai-Tibetan Plateau. We found that lower soil moisture reduced the fractional concentrations of cyclic bGDGTs and thus the cyclisation ratio of branched tetraethers (CBT) index correlated negatively with SWC and MAP, suggesting that soil moisture is an important factor controlling bGDGT distributions in soils in this arid and semi-arid region. Two subgroups of bGDGTs were assigned on the basis of cluster analysis, and bGDGT indices behaved differently in the two groups, hinting at different biosynthetic mechanisms for bGDGTs under different environmental conditions. Real time PCR results showed that nirS and nirK genes correlated significantly with the concentration of bGDGTs, suggesting that the nirS- or nirK-encoding bacteria involved in denitrification might potentially be an additional biological source for soil bGDGTs (besides Acidobacteria). Moreover, our results also support the application of new indices based on 5-methyl bGDGTs and 6-methyl bGDGTs in reconstructing past temperature and pH variations in this region.