Matrine inhibits the growth of retinoblastoma cells (SO-Rb50) by decreasing proliferation and inducing apoptosis in a mitochondrial pathway
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- 作者：Qingliang Shao (1)
Xiaxia Zhao (1)
Li Yao (1) - 关键词：Matrine ; Apoptosis ; Mitochondrial transmembrane potential ; Retinoblastoma ; Caspase
- 刊名：Molecular Biology Reports
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：41
- 期：5
- 页码：3475-3480
- 全文大小：
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Xiaxia Zhao (1)
Li Yao (1)
1. Department of Pediatrics, The Second Affiliated Hospital of Harbin Medical University, 148 Baojian Street, Nangang District, Harbin, 150081, Heilongjiang, China - ISSN：1573-4978
文摘
Matrine, one of the main active components of extracts from the dry roots of Sophora flavescens, has potent anti-tumor activity in vitro and in vivo. Here, we investigated the apoptosis in matrine-treated retinoblastoma cells. The results showed that matrine could inhibit cell proliferation and induce apoptosis in a dose- and time-dependent manner. Further investigation revealed that a disruption of mitochondrial transmembrane potential and an up-regulation of reactive oxygen species in matrine-treated cells. By western blot analysis, we found that the up-regulation of cleaved Apaf-1, cleaved caspase-3, cleaved caspase-9, cleaved caspase-7, Bax/Bcl-2, varying with different concentration of matrine. These protein interactions may play a pivotal role in the regulation of apoptosis. Taken together, these results overall indicate that matrine could be used as an effective anti-tumor agent in therapy of retinoblastoma targets the caspase-dependent signaling pathway.