A repressor motif-containing poplar R3 MYB-like transcription factor regulates epidermal cell fate determination and anthocyanin biosynthesis in Arabidopsis
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- 作者：Qingnan Hu ; Li Yang ; Shanda Liu ; Limei Zhou ; Xutong Wang…
- 关键词：Anthocyanin ; Arabidopsis thaliana ; Root hair ; Transcription factor ; Trichome ; Populus trichocarpa ; PtrRML1
- 刊名：Journal of Plant Biology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：59
- 期：5
- 页码：525-535
- 全文大小：1,782 KB
- 刊物主题：Plant Sciences; Plant Breeding/Biotechnology; Plant Genetics & Genomics; Plant Systematics/Taxonomy/Biogeography; Plant Ecology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1867-0725
- 卷排序：59
文摘
Single-repeat R3 MYB transcription factors (R3 MYBs) regulate epidermal cell fate determination in Arabidopsis through a lateral inhibition mechanism. Previously we have shown that poplar R3 MYB genes regulate trichome formation when expressed in Arabidopsis. Here we report the identification and functional characterization of a poplar R3 MYB-like protein, Populus trichocarpa R3 MYB-LIKE1 (PtrRML1). So far all the MYB transcription factors identified have a highly conserved N-terminal DNA-binding domain composed of MYB repeats and a highly variable C-terminal domain. However, PtrRML1 has a single R3 MYB repeat at its C-terminal and an LxLxL repressor motif-containing N-terminal, and with amino acids about twice of that of the known R3 MYBs. PtrRML1 is localized in nuclear in transgenic Arabidopsis plants expressing PtrRML1-GFP. PtrRML1 repressed reporter gene expression in protoplasts, and it interacted with GL3 in plant cells. Expression of PtrRML1 in Arabidopsis resulted in glabrous phenotypes, increased number of root hairs, and decreased anthocyanin accumulation. Consist with these observation, the expression levels of some MBW component genes and some of their target genes involved in the regulation of epidermal cell fate determination and anthocyanin biosynthesis, including TRY, CPC, ETC1, GL1, GL2, DFR, UF3GT and TT8 were reduced in the transgenic plants.