High density methylation QTL analysis in human blood via next-generation sequencing of the methylated genomic DNA fraction
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- 作者：Joseph L. McClay ; Andrey A. Shabalin ; Mikhail G. Dozmorov…
- 关键词：DNA methylation ; next ; generation sequencing ; GWAS ; single nucleotide polymorphism ; quantitative trait loci ; chromatin states
- 刊名：Genome Biology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：16
- 期：1
- 全文大小：2,346 KB
- 刊物主题：Animal Genetics and Genomics; Human Genetics; Plant Genetics & Genomics; Microbial Genetics and Genomics; Fungus Genetics; Bioinformatics;
- 出版者：BioMed Central
- ISSN：1465-6906
文摘
Background Genetic influence on DNA methylation is potentially an important mechanism affecting individual differences in humans. We use next-generation sequencing to assay blood DNA methylation at approximately 4.5 million loci, each comprising 2.9 CpGs on average, in 697 normal subjects. Methylation measures at each locus are tested for association with approximately 4.5 million single nucleotide polymorphisms (SNPs) to exhaustively screen for methylation quantitative trait loci (meQTLs).