An important role of interleukin-10 in counteracting excessive immune response in HT-29 cells exposed to Clostridium butyricum
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- 作者：Quanxin Gao (1) (2)
Lili Qi (1)
Tianxing Wu (1) (2)
Jinbo Wang (1) - 刊名：BMC Microbiology
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：12
- 期：1
- 全文大小：323KB
- 参考文献：1. Jia W, Li H, Zhao L, Nicholson JK: Gut microbiota: a potential new territory for drug targeting. / Nat Rev Drug Discov 2008, 7:123-29. CrossRef
2. Haller D, Bode C, Hammes WP, Pfeifer AM, Schiffrin EJ, Blum S: Nonpathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. / Gut 2000, 47:79-7. CrossRef
3. McCracken VJ, Chun T, Baldeon ME, Ahrne S, Molin G, Mackie RI, Gaskins HR: TNF-alpha sensitizes HT-29 colonic epithelial cells to intestinal lactobacilli. / Exp Biol Med 2002, 227:665-70.
4. Shanahan F: Probiotics in inflammatory bowel disease - therapeutic rationale and role. / Adv Drug Deliv Rev 2004, 56:809-18. CrossRef
5. Sartor RB: Targeting enteric bacteria in treatment of inflammatory bowel diseases: why, how, and when. / Curr Opin Gastroenterol 2003, 19:358-65. CrossRef
6. Kuhn R, Lohler J, Rennick D, Rajewsky K, Muller W: Interleukin-10-deficient mice develop chronic enterocolitis. / Cell 1993,75(2):263-74. CrossRef
7. Lavasani S, Dzhambazov B, Nouri M, F?k F, Buske S, Molin G, Thorlacius H, Alenfall J, Jeppsson B, Westr?m B: A novel Probiotic mixture exerts a therapeutic effect on experimental autoimmuneencephalomyelitis mediated by IL-10 producing regulatory T cells. / PLoS One 2010,5(2):e9009. CrossRef
8. Mengheri E: Health, probiotics, and inflammation. / J Clin Gastroenterol 2008, 42:s177-s178. CrossRef
9. Ito I, Hayashi T, Iguchi A, Endo H, Nakao M, Kato S, Nabeshima T, Ogura Y: Effects of administration of Clostridium butyricum to patients receiving long-term tube feeding. / Jpn J Geriat 1997, 34:298-04. CrossRef
10. Pan X, Wu T, Zhang L, Song Z, Tang H, Zhao Z: In vitro evaluation on adherence and antimicrobial properties of a candidate probiotic Clostridium butyricum CB2 for farmed fish. / J Appl Microbiol 2008, 105:1623-629. CrossRef
11. Li YY, Chang JW, Hsieh LL, Yeh KY: Neutralization of interleukin (IL)-10 released by monocytes/macrophages enhances the up-regulatory effect of monocyte/macrophage-derived IL-6 on expressions of IL-6 and MUC1, and migration in HT-29 colon cancer cells. / Cell Immunol 2010,265(2):164-71. CrossRef
12. Wang JB, Qi LL, Zheng SD, Wang HZ, Wu TX: Curcumin suppresses PPARδ expression and related genes in HT-29 cells. / World J Gastroenterol 2009, 15:1346-352. CrossRef
13. Jin S, Zhang QY, Kang XM, Wang JX, Zhao WH: Daidzein induces MCF-7 breast cancer cell apoptosis via the mitochondrial pathway. / Ann Oncol 2010, 21:263-68. CrossRef
14. Jia YD, Lin JX, Mi YL, Zhang CQ: Quercetin attenuates cadmium-induced oxidative damage and apoptosis in granulosa cells from chicken ovarian follicles. / Reprod Toxicol 2011,31(4):477-85. CrossRef
15. Christensen HR, Fr?kiaer H, Pestka JJ: Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. / J Immunol 2002,168(1):171-78.
16. Altonsy MO, Andrews SC, Tuohy KM: Differential induction of apoptosis in human colonic carcinoma cells (Caco-2) by Atopobium, and commensal, probiotic and enteropathogenic bacteria: mediation by the mitochondrial pathway. / Int J Food Microbiol 2010,137(2-):190-03. CrossRef
17. Zhang WJ, Li BH, Yang XZ, Li PD, Yuan Q, Liu XH, Xu SB, Zhang Y, Yuan J, Gerhard GS, Masker KK, Dong C, Koltun WA, Chorney MJ: IL-4-induced Stat6 activities affect apoptosis and gene expression in breast cancer cells. / Cytokine 2008,42(1):39. CrossRef
18. Fiorentino DF, Zlotnik A, Mosmann TR, Howard M, O’Garra A: IL-10 inhibits cytokine production by activated macrophages. / J Immunol 1991, 147:3815-822.
19. Poe JC, Wagner DH, Miller RW, Stout RD, Suttles J: IL-4 and IL-10 modulation of CD40-mediated signaling of monocyte IL-1b synthesis and rescue from apoptosis. / J Immunol 1997, 159:846-52.
20. Rennick DM, Fort MM: Lesson from genetically engineered animal models XII: IL-10- deficient mice and intestinal inflammation. / Am J Physiol 2000, 278:g829-g833.
21. Gazzinelli RT, Wysocka M, Hieny S, Scharton-Kersten T, Cheever A, Kuhn R, Muller W, Trinchieri G, Sher A: In the absence of endogenous IL-10, mice acutely infected with Toxoplasma gondii succumb to a lethal immune response dependent on CD4+ T cells and accompanied by overproduction of IL-12, IFN-c and TNF-a. / J Immunol 1996, 157:798-05.
22. Akemi I, Tatsuichiro S, Kimitoshi K, Shigeaki M, Toshiki Ua, Satoshi Mo, Hiromi S, Taeko H, Yoshinori U: Anti-inflammatory activity of probiotic Bifidobacterium: enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells. / World J Gastroenterol 2008,14(16):2511-516. CrossRef
23. Smits HH, Engering A, van der Kleij D, de Jong EC, Schipper K, van Capel TM, Zaat BA, Yazdanbakhsh M, Wierenga EA, van Kooyk Y, Kapsenberg ML: Selective probiotic bacteria induce IL-10-producing regulatory T cells in vitro by modulating dendritic cell function through dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin. / J Allergy Clin Immunol 2005,115(6):1260-267. CrossRef
24. Kim SY, Kim JY, Kim SH, Bae HJ, Yi H, Yoon SH, Koo BS, Kwon M, Cho JY, Lee CE, Hong S: Surfactin from Bacillus subtilis displays antiproliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression. / FEBS Lett 2007, 581:865-71. CrossRef
25. Koonin EV, Aravind L: Origin and evolution of eukaryotic apoptosis: the bacterial connection. / Cell Death Differ 2002, 9:394-04. CrossRef
26. Hooper LV, Gordon JI: Commensal host-bacterial relationships in the gut. / Science 2001, 292:1114-118. CrossRef - 作者单位：Quanxin Gao (1) (2)
Lili Qi (1)
Tianxing Wu (1) (2)
Jinbo Wang (1)
1. Key Laboratory for Molecular Design and Nutrition Engineering, Ningbo Institute of Technology, Zhejiang University, No. 1 Qianhunan Road, Ningbo, 315100, China
2. College of Animal Sciences, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou, 310000, China - ISSN：1471-2180
文摘
Background Clostridium butyricum has become increasingly important in preventing and treating intestinal inflammation. In the intestine it may increase the resistance of the gut to pathogen invasion via inducing the secretion of anti-inflammatory cytokines. Interleukin 10 (IL-10) plays a central role in preventing certain inflammatory diseases by down-regulating inflammatory cascades. In a previous study, we observed that the level of IL-10 mRNA was modulated by C. butyricum. The aim of this study was to investigate whether C. butyricum achieves its beneficial effects through IL-10. Results We treated HT-29 cells with anti-IL-10 (IL-10 antibody) or siIL-10 (IL-10 small interfering RNA) to disrupt IL-10. In both cases, the effects of C. butyricum-induced NF-κB activation and IL-8 expression were enhanced. We also found that neutralization or knockdown of IL-10 could induce apoptosis and necrosis of HT-29 cells treated with C. butyricum compared with control cells. Conclusions These findings show that IL-10 serves an important role in C. butyricum-mediated immune protection, and in host recognition of C. butyricum.