Adenovirus-mediated IL-24 confers radiosensitization to human lung adenocarcinoma in vitro and in vivo

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• 作者：Shi-Ying Zheng ; Jin-Feng Ge ; Jun Zhao ; Dong Jiang ; Fang Li
• 关键词：IL ; 24 ; Radiotherapy ; Adenocarcinoma ; Radiosensitization
• 刊名：Molecular Biology Reports
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：42
• 期：6
• 页码：1069-1080
• 全文大小：1,654 KB
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• 作者单位：Shi-Ying Zheng (1)
  Jin-Feng Ge (1)
  Jun Zhao (1)
  Dong Jiang (1)
  Fang Li (2)
  
  1. Department of Thoracocardiac Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu, China
  2. Department of Human Anatomy, Histology and Embryology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215007, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

The current paper aims to study the effect of adenovirus-mediated IL-24 (Ad-IL-24) on human lung adenocarcinoma in vitro and in vivo and determine its possible mechanism of action. The growth-suppressing and apoptosis-inducing effects of Ad-IL-24, radiotherapy, and Ad-IL-24+?radiotherapy (hereinafter referred to as the joint group) on SPC-A1 lung carcinoma cells were assessed by using 3-(4,5-dimethyliazolyl-2)-2,5-diphnyltetrazolium bromide and flow cytometry. A human lung model was established with SPC-A1 cells in nude mice. Groups of mice were subjected to multi-point injections to their tumors. Gross tumor volumes were measured dynamically. The ratios of tumor suppression and radiosensitization effect were evaluated according to the method of probability sum Q values. The expressions of Bax, Bcl-2, Survivin, and Caspase-3 in tumor samples were detected by immunohistochemistry. The ratios of inhibition and apoptosis in the joint group were higher than those in the individual Ad-IL-24 and radiotherapy groups. In vitro, the joint group suppressed tumor growth conspicuously, showing a weight inhibition rate of about 64?%. The expressions of FasL, Bax and Caspase-3 were upregulated in the joint group, while the expressions of Cox,Bcl-2,VEGF,CD34 and Survivin were downregulated. The current study proves that Ad-IL-24 suppresses growth of SPC-A1 cells both in vitro and in vivo. Its functions appear to be related to cell apoptosis and antiangiogenesis.