Antioxidant and Antiproliferative Activity of Diospyros lotus L. Extract and Isolated Compounds
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- 作者：Monica Rosa Loizzo (1)
Ataa Said (2)
Rosa Tundis (1)
Usama W. Hawas (3)
Khaled Rashed (2)
Federica Menichini (1)
Natale Giuseppe Frega (4)
Francesco Menichini (1) - 关键词：Diospyros lotus L. ; Phenolic compounds ; Antioxidant activity ; Antiproliferative
- 刊名：Plant Foods for Human Nutrition
- 出版年：2009
- 出版时间：December 2009
- 年：2009
- 卷：64
- 期：4
- 页码：264-270
- 全文大小：140KB
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Ataa Said (2)
Rosa Tundis (1)
Usama W. Hawas (3)
Khaled Rashed (2)
Federica Menichini (1)
Natale Giuseppe Frega (4)
Francesco Menichini (1)
1. Department of Pharmaceutical Sciences, Faculty of Pharmacy, Nutrition and Health Sciences, University of Calabria, 87036, Rende, CS, Italy
2. Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt
3. Phytochemistry and Plant Systematic Department, National Research Centre, Dokki, Giza, Egypt
4. Department of Food Science, Politecnica delle Marche University, via Brecce Bianche, 60131, Ancona, Italy
文摘
The object of the study was to determine the chemical composition of Diospyros lotus L. extract and their antioxidant and antiproliferative properties. Eight compounds were isolated from D. lotus and identified as gallic acid, methylgallate, ellagic acid, kaempferol, quercetin, myricetin, myricetin 3-O-尾-glucuronide, and myricetin-3-O-伪-rhamnoside. D. lotus extract tested in different in vitro systems (DPPH, ABTS, FRAP, and Fe2+ chelating activity assay) showed significant antioxidant activity. The potential antiproliferative properties of D. lotus extract and isolated compounds against nine human cancer cell lines such as COR-L23, CaCo-2, C32, ACHN, A375, A549, Huh-7D12, MCF-7, and LNCaP were investigated in vitro by SRB assay. D. lotus extract demonstrated the highest inhibitory activity against COR-L23 with an IC50 value of 12.2聽渭g/ml. Among identified hydrolysable tannins, ellagic acid evidenced strong antiproliferative activity against both C32 and A375 cells with IC50 values of 0.8 and 4.1聽渭g/ml, respectively. Interesting results were observed, also, with gallic acid that showed the highest cytotoxic activity against CaCo-2 (IC50 2.6聽渭g/ml). Overall, the results of this study suggest that D. lotus displays a good antioxidant activity and has antiproliferative effects. Both activities are related to identified phenolic compounds.