Toll-like receptors in the brain of mice following infection with Acanthamoeba spp.

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• 作者：Agnieszka Wojtkowiak-Giera ; Monika Derda…
• 关键词：TLR2 ; TLR4 ; Toll ; like receptors ; Q ; PCR ; Immunohistochemistry ; Acanthamoeba spp. ; Brain ; Mouse
• 刊名：Parasitology Research
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：115
• 期：11
• 页码：4335-4344
• 全文大小：1,017 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955
• 卷排序：115

文摘

The Toll-like receptors (TLRs) of the innate immune system play an important role in the recognition of pathogens such as bacteria, viruses, fungi, and parasites. In this study, we examined the changes in the level of expression of TLR2 and TLR4 mRNA and protein in the brains of mice infected with Acanthamoeba spp. The Acanthamoeba strains were isolated from a patient with Acanthamoeba keratitis (AK) (Ac55) and Malta Lake (Ac43). In the brain isolated from mice at 2 days post-infection (dpi) with Acanthamoeba strains Ac55 and Ac43, mRNAs for TLR2 and TLR4 were significantly more strongly expressed in comparison with the uninfected mice. In Acanthamoeba-infected mice, TLR2 and TLR4 expression was detected in neurons, glial cells, and endothelial cells within the neocortex. These receptors showed more intense expression in ependymocytes of the choroid plexus of infected mice at 2 dpi. Increased levels of TLR2 and TLR4 mRNA expression in infected mice suggest the involvement of these TLRs in the recognition of Acanthamoeba spp. pathogen-associated molecular patterns (PAMPs).