Knockdown of CLIC4 enhances ATP-induced HN4 cell apoptosis through mitochondrial and endoplasmic reticulum pathways

详细信息    查看全文

• 作者：Haowei Xue ; Jinsen Lu ; Renxiang Yuan ; Jinli Liu ; Yehai Liu ; Kaile Wu…
• 关键词：Chloride intracellular channel 4 ; Head and neck squamous carcinoma ; Apoptosis ; Mitochondrial membrane potential ; Endoplasmic reticulum stress ; Adenosine triphosphate
• 刊名：Cell & Bioscience
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：1
• 全文大小：1,511 KB
• 参考文献：1.Machiels JP, Lambrecht M, Hanin FX, Duprez T, Gregoire V, Schmitz S, et al. Advances in the management of squamous cell carcinoma of the head and neck. F1000Prime Rep. 2014;6:44.PubMedCentral CrossRef PubMed
  2.Behera M, Owonikoko TK, Kim S, Chen Z, Higgins K, Ramalingam SS, et al. Concurrent therapy with taxane versus non-taxane containing regimens in locally advanced squamous cell carcinomas of the head and neck (SCCHN): a systematic review. Oral Oncol. 2014;50(9):888–94.CrossRef PubMed
  3.Pancari P, Mehra R. Systemic therapy for squamous cell carcinoma of the head and neck. Surg Oncol Clin N Am. 2015;24(3):437–54.CrossRef PubMed
  4.Lehen’kyi V, Shapovalov G, Skryma R, Prevarskaya N. Ion channnels and transporters in cancer. 5. Ion channels in control of cancer and cell apoptosis. Am J Physiol Cell Physiol. 2011;301(6):C1281–9.CrossRef PubMed
  5.Peretti M, Angelini M, Savalli N, Florio T, Yuspa SH, Mazzanti M. Chloride channels in cancer: Focus on chloride intracellular channel 1 and 4 (CLIC1 AND CLIC4) proteins in tumor development and as novel therapeutic targets. Biochim Biophys Acta. 2015;1848((10 Pt B)):2523–31.CrossRef PubMed
  6.Jentsch TJ, Stein V, Weinreich F, Zdebik AA. Molecular structure and physiological function of chloride channels. Physiol Rev. 2002;82(2):503–68.CrossRef PubMed
  7.Fernandez-Salas E, Suh KS, Speransky VV, Bowers WL, Levy JM, Adams T, et al. mtCLIC/CLIC4, an organellular chloride channel protein, is increased by DNA damage and participates in the apoptotic response to p53. Mol Cell Biol. 2002;22(11):3610–20.PubMedCentral CrossRef PubMed
  8.Mutoh M, Suh KS, Gerdes M, Yuspa SH. CLIC4, an intracellular chloride channel protein, is a novel molecular target for cancer therapy. J Investig Dermatol Symp Proc. 2005;10:105–9.CrossRef PubMed
  9.Zhong J, Kong X, Zhang H, Yu C, Xu Y, Kang J, et al. Inhibition of CLIC4 enhances autophagy and triggers mitochondrial and ER stress-induced apoptosis in human glioma U251 cells under starvation. PLoS One. 2012;7(6):e39378.PubMedCentral CrossRef PubMed
  10.Xu Y, Kang J, Yuan Z, Li H, Su J, Li Y, et al. Suppression of CLIC4/mtCLIC enhances hydrogen peroxide-induced apoptosis in C6 glioma cells. Oncol Rep. 2013;29(4):1483–91.PubMed
  11.Tang HY, Beer LA, Tanyi JL, Zhang R, Liu Q, Speicher DW. Protein isoform-specific validation defines multiple chloride intracellular channel and tropomyosin isoforms as serological biomarkers of ovarian cancer. J Proteomics. 2013;89:165–78.PubMedCentral CrossRef PubMed
  12.Suh KS, Malik M, Shukla A, Ryscavage A, Wright L, Jividen K, et al. CLIC4 is a tumor suppressor for cutaneous squamous cell cancer. Carcinogenesis. 2012;33(5):986–95.PubMedCentral CrossRef PubMed
  13.Zheng DL, Huang QL, Zhou F, Huang QJ, Lin JY, Lin X. PA28beta regulates cell invasion of gastric cancer via modulating the expression of chloride intracellular channel 1. J Cell Biochem. 2012;113(5):1537–46.PubMed
  14.Suh KS, Mutoh M, Gerdes M, Crutchley JM, Mutoh T, Edwards LE, et al. Antisense suppression of the chloride intracellular channel family induces apoptosis, enhances tumor necrosis factor {alpha}-induced apoptosis, and inhibits tumor growth. Cancer Res. 2005;65(2):562–71.PubMed
  15.Suh KS, Crutchley JM, Koochek A, Ryscavage A, Bhat K, Tanaka T, et al. Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. Clin Cancer Res. 2007;13(1):121–31.CrossRef PubMed
  16.Selzner N, Selzner M, Graf R, Ungethuem U, Fitz JG, Clavien PA. Water induces autocrine stimulation of tumor cell killing through ATP release and P2 receptor binding. Cell Death Differ. 2004;11(Suppl 2):S172–80.CrossRef PubMed
  17.Humphreys BD. SAPK/JNK activation and apoptotic induction by the macrophage P2X7 nucleotide receptor. J Biol Chem. 2000;275:26792–8.PubMed
  18.Walia V, Kakar S, Elble R. Micromanagement of the mitochondrial apoptotic pathway by p53. Front Biosci (Landmark Ed). 2011;16:749–58.CrossRef
  19.Ly JD, Grubb DR, Lawen A. The mitochondrial membrane potential (deltapsi(m)) in apoptosis; an update. Apoptosis. 2003;8(2):115–28.CrossRef PubMed
  20.Perveen S, Yang JS, Ha TJ, Yoon SH. Cyanidin-3-glucoside inhibits ATP-induced intracellular free Ca(2+) concentration, ROS formation and mitochondrial depolarization in PC12 cells. Korean J Physiol Pharmacol. 2014;18(4):297–305.PubMedCentral CrossRef PubMed
  21.Contreras L, Drago I, Zampese E, Pozzan T. Mitochondria: the calcium connection. Biochim Biophys Acta. 2010;1797(6–7):607–18.CrossRef PubMed
  22.Bernardi P, Rasola A. Calcium and cell death: the mitochondrial connection. Subcell Biochem. 2007;45:481–506.CrossRef PubMed
  23.Yang Y, Zhu J, Wang X, Xue N, Du J, Meng X, et al. Contrasting Patterns of Agonist-induced Store-operated Ca2+ Entry and Vasoconstriction in Mesenteric Arteries and Aorta With Aging. J Cardiovasc Pharmacol. 2015;65(6):571–8.PubMedCentral CrossRef PubMed
  24.Srinivasan V, Sundar Rajan S, Balasubramanyam M, Tatu U. Endoplasmic reticulum (ER) stress & diabetes. Indian J Med Res. 2007;125:411–24.PubMed
  25.Zhu W, Wang X, Zhou Y, Wang H. C2-ceramide induces cell death and protective autophagy in head and neck squamous cell carcinoma cells. Int J Mol Sci. 2014;15(2):3336–55.PubMedCentral CrossRef PubMed
  26.Michaud M, Martins I, Sukkurwala AQ, Adjemian S, Ma Y, Pellegatti P, et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 2011;334(6062):1573–7.CrossRef PubMed
  27.Wong WW, Puthalakath H. Bcl-2 family proteins: the sentinels of the mitochondrial apoptosis pathway. IUBMB Life. 2008;60(6):390–7.CrossRef PubMed
  28.Ranganathan V, Murphy KM, Farnsworth ML, Kavallaris M, Lock RB. BCL-2 inhibits BAX translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells. Cell Death Differ. 2002.
  29.Gogol P, Trzcińska M, Bryła M. Motility, mitochondrial membrane potential and ATP content of rabbit spermatozoa stored in extender supplemented with GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide. Pol J Vet Sci. 2014;17(4):571–5.PubMed
  30.Wang WA, Groenendyk J, Michalak M. Endoplasmic reticulum stress associated responses in cancer. Biochim Biophys Acta. 2014;1843(10):2143–9.CrossRef PubMed
  31.Scorrano L, Oakes SA, Opferman JT, Cheng EH, Sorcinelli MD, Pozzan T, et al. BAX and BAK regulation of endoplasmic reticulum Ca2+: a control point for apoptosis. Science. 2003;300(5616):135–9.CrossRef PubMed
  32.Oakes SA, Opferman JT, Pozzan T, Korsmeyer SJ, Scorrano L. Regulation of endoplasmic reticulum Ca2+ dynamics by proapoptotic BCL-2 family members. Biochem Pharmacol. 2003;66(8):1335–40.CrossRef PubMed
  33.Fels DR, Koumenis C. The PERK/eIF2α/ATF4 module of the UPR in hypoxia resistance and tumor growth. Cancer Biol Ther. 2006;5:723–8.CrossRef PubMed
  34.Yoshida H, Matsui T, Yamamoto A, Okada T, Mori K. XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001;107:881–91.CrossRef PubMed
  35.Jiang HY, Wek RC. Phosphorylation of the alpha-subunit of the eukaryotic initiation factor-2 (eIF2alpha) reduces protein synthesis and enhances apoptosis in response to proteasome inhibition. J Biol Chem. 2005;280(14):14189–202.CrossRef PubMed
  36.Oyadomari S, Mori M. Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell Death Differ. 2004;11(4):381–9.CrossRef PubMed
  37.World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013;310:2191–4.CrossRef
  38.Grimes DA, Hubacher D, Nanda K, Schulz KF, Moher D, Altman DG. The good clinical practice guideline: a bronze standard for clinical research. Lancet. 2005;366(9480):172–4.CrossRef PubMed
  39.Kim SY, Chu KC, Lee HR, Lee KS, Carey TE. Establishment and characterization of nine new head and neck cancer cell lines. Acta Otolaryngol. 1997;117(5):775–84.CrossRef PubMed
  40.Ma Y, Zhang P, Li J, Lu J, Ge J, Zhao Z, et al. Epoxyeicosatrienoic acids act through TRPV4-TRPC1-KCa1.1 complex to induce smooth muscle membrane hyperpolarization and relaxation in human internal mammary arteries. Biochim Biophys Acta. 2015;1852(3):552–9.CrossRef PubMed
  41.Chen J, Liao W, Gao W, Huang J, Gao Y. Intermittent hypoxia protects cerebral mitochondrial function from calcium overload. Acta Neurol Belg. 2013;113(4):507–13.CrossRef PubMed
  42.Shen B, Zhu J, Zhang J, Jiang F, Wang Z, Zhang Y, et al. Attenuated mesangial cell proliferation related to store-operated Ca2+ entry in aged rat: the role of STIM 1 and Orai 1. Age (Dordr). 2013;35(6):2193–202.PubMedCentral CrossRef PubMed
  
• 作者单位：Haowei Xue (1)
  Jinsen Lu (2)
  Renxiang Yuan (2)
  Jinli Liu (3)
  Yehai Liu (4)
  Kaile Wu (4)
  Jing Wu (4)
  Juan Du (2)
  Bing Shen (2)
  
  1. Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China
  2. Department of Physiology, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China
  3. Department of Dermatology, Anhui Provincial Hospital, Hefei, 230001, Anhui, China
  4. Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China
  
• 刊物主题：Cell Biology; Microbiology;
• 出版者：BioMed Central
• ISSN：2045-3701

文摘

Background Human head and neck squamous carcinoma is the 6th most prevalent carcinoma worldwide. Although many novel therapies have been developed, the clinical treatment for patients remains non-ideal. Chloride intracellular channel 4 (CLIC4), one of the seven members of the CLIC family, is a newly found Cl− channel that participates in various biological processes, including cellular apoptosis and differentiation. Accumulating evidence has revealed the significant role of CLIC4 in regulating the apoptosis of different cancer cells. Here, we investigated the functional role of CLIC4 in the apoptosis of HN4 cells, a human head and neck squamous carcinoma cell line.