ACE inhibition modulates transforming growth factor-? receptors in the young rat
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- 作者：Nam Soo Kang ; Hyung Eun Yim ; In Sun Bae ; Jeong Hoon Choi ; Byung Min Choi ; Kee Hwan Yoo ; Young Sook Hong ; Joo Won Lee and Soon Kyum Kim
- 关键词：Renin ; angiotensin system ; Angiotensin converting enzyme inhibitors ; Transforming growth factor 1 ; Transforming growth factor receptor
- 刊名：Pediatric Nephrology
- 出版年：2003
- 出版时间：September 2003
- 年：2003
- 卷：18
- 期：9
- 页码：865-871
- 全文大小：593 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Pediatrics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-198X
文摘
The renin-angiotensin system plays an important role in renal growth and development. Exposure of the neonate to angiotensin converting enzyme (ACE) inhibitors increases mortality and results in growth retardation and abnormal renal development. It has been demonstrated that ACE inhibition in the developing kidney reduces the renal expression of growth factors, which may account for renal growth impairment. This study was designed to investigate the relationship between renal growth impairment and the expression of transforming growth factor-1 (TGF-1), TGF- receptor I [TRI, activin-like kinase (ALK)-1 and ALK-5], and TGF- receptor II (TRII). Newborn rat pups were treated with enalapril (30 mg/kg per day) or vehicle for 7 days, and kidneys were removed for Western blotting of TGF-1, ALK-1, ALK-5, and TRII, and for RT-PCR of ALK-5 and TRII. TGF-1, ALK-1, ALK-5, and TRII were also detected by immunohistochemistry. Enalapril treatment resulted in an increased mortality (30.4%) by day 7, and reduced body weight and kidney weight (P<0.05 versus vehicle). Enalapril decreased renal TGF-1, ALK-1, and ALK-5 protein expression (P<0.05). Also, enalapril decreased ALK-5 mRNA expression (P<0.05). TRII expression was not changed by enalapril treatment. These results indicate that ACE inhibition in the developing kidney decreases TGF-1, ALK-1, and ALK-5 expression, which may account for renal growth impairment. TRII may not be modulated by ACE inhibition in the developing kidney.