Molecular genetic features of 5S rDNA nontranscribed spacer in Hippophae rhamnoides L.

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• 作者：O. S. Alexandrov ; A. V. Evtukhov…
• 关键词：5S rDNA ; nontranscribed spacer ; microsatellite locus ; Hippophae rhamnoides L. ; Elaeagnus angustifolia L. ; Calligonum mongolicum Turcz
• 刊名：Moscow University Biological Sciences Bulletin
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：71
• 期：4
• 页码：231-234
• 全文大小：
• 刊物主题：Biochemistry, general; Cell Biology; Life Sciences, general; Plant Sciences; Zoology;
• 出版者：Allerton Press
• ISSN：1934-791X
• 卷排序：71

文摘

Amplification of sea buckthorn Hippophae rhamnoides L. 5S rDNA nontranscribed spacer with coding border anneal primers showed existence of a single fragment. The fragment was cloned and sequenced. It was shown that length of the Hippophae rhamnoides L. 5S rDNA nontranscribed spacer is 807 bp. Analysis of the sequence allowed to detect a high homology with early described microsatellite locuses of Hippophae rhamnoides L., russian olive Elaeagnus angustifolia L., and Calligonum mongolicum Turcz. that include a (GA)₉ motif. These results may be useful to study a ribosomal RNA gene organization.