Hippophae rhamnoides N-glycoproteome analysis: a small step towards sea buckthorn proteome mining

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• 作者：Yaiphabi Sougrakpam ; Renu Deswal
• 关键词：N ; glycoproteins ; Affinity chromatography ; Stains all ; Secretory
• 刊名：Physiology and Molecular Biology of Plants
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：22
• 期：4
• 页码：473-484
• 全文大小：
• 刊物主题：Plant Sciences; Plant Physiology; Biological and Medical Physics, Biophysics; Cell Biology;
• 出版者：Springer India
• ISSN：0974-0430
• 卷排序：22

文摘

Hippophae rhamnoides is a hardy shrub capable of growing under extreme environmental conditions namely, high salt, drought and cold. Its ability to grow under extreme conditions and its wide application in pharmaceutical and nutraceutical industry calls for its in-depth analysis. N-glycoproteome mining by con A affinity chromatography from seedling was attempted. The glycoproteome was resolved on first and second dimension gel electrophoresis. A total of 48 spots were detected and 10 non-redundant proteins were identified by MALDI–TOF/TOF. Arabidopsis thaliana protein disulfide isomerase-like 1-4 (ATPDIL1-4) electron transporter, protein disulphide isomerase, calreticulin 1 (CRT1), glycosyl hydrolase family 38 (GH 38) protein, phantastica, maturase k, Arabidopsis trithorax related protein 6 (ATXR 6), cysteine protease inhibitor were identified out of which ATXR 6, phantastica and putative ATPDIL1-4 electron transporter are novel glycoproteins. Calcium binding protein CRT1 was validated for its calcium binding by stains all staining. GO analysis showed involvement of GH 38 and ATXR 6 in glycan and lysine degradation pathways. This is to our knowledge the first report of glycoproteome analysis for any Elaeagnaceae member.