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Establishment and characterization of a cell line from the mosquito Culex tritaeniorhynchus (Diptera: Culicidae)
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  • 作者：Ryusei Kuwata (1)
    Keita Hoshino (1)
    Haruhiko Isawa (1)
    Yoshio Tsuda (1)
    Shigeru Tajima (2)
    Toshinori Sasaki (1)
    Tomohiko Takasaki (2)
    Mutsuo Kobayashi (1)
    Kyoko Sawabe (1)
  • 关键词：Mosquito cell line ; Japanese encephalitis virus ; Dengue virus ; Flaviviridae ; Arbovirus
  • 刊名：In Vitro Cellular & Developmental Biology - Animal
  • 出版年：2012
  • 出版时间：June 2012
  • 年：2012
  • 卷：48
  • 期：6
  • 页码：369-376
  • 全文大小：599KB
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  • 作者单位：Ryusei Kuwata (1)
    Keita Hoshino (1)
    Haruhiko Isawa (1)
    Yoshio Tsuda (1)
    Shigeru Tajima (2)
    Toshinori Sasaki (1)
    Tomohiko Takasaki (2)
    Mutsuo Kobayashi (1)
    Kyoko Sawabe (1)

    1. Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan
    2. Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan
文摘
We established a continuous cell line from the embryo of the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae), a known major vector of the Japanese encephalitis virus (family Flaviviridae, genus Flavivirus) in Asia. The cell line, designated NIID-CTR, was serially subcultured in VP-12 medium supplemented with 10?% heat-inactivated fetal bovine serum (FBS). It continued to grow for more than 60 passages over a 750-d period. The NIID-CTR cell line mainly comprised two morphologically distinct types of cells with adhesive properties: spindle-shaped and round cells. Most of the NIID-CTR cells at the 45th passage were diploid (2n--). The growth kinetics of the NIID-CTR cells was significantly affected by the FBS concentration in the medium. The population doubling time of the NIID-CTR cells was 20?h in the presence of 10?% FBS and 76?h in its absence. The DNA sequence of the mitochondrial cytochrome oxidase I gene confirmed that the NIID-CTR cell line was derived from C. tritaeniorhynchus. The cells were highly susceptible to Japanese encephalitis and Dengue viruses, thus providing a valuable tool for the study of mosquito-borne flaviviruses.

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