Expression of OsAMT1 (1.1-1.3) in rice varieties differing in nitrogen accumulation
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- 作者：S. P. Zhao (1) (2)
X. Z. Ye (1)
W. M. Shi (3) - 关键词：Oryza sativa ; OsAMT1 ; transporter ; nitrogen
- 刊名：Russian Journal of Plant Physiology
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：61
- 期：5
- 页码：707-713
- 全文大小：184 KB
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X. Z. Ye (1)
W. M. Shi (3)
1. Zhejiang Province Key Lab for Food Safety, Institute of Quality and Standard for Agroproducts, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China
2. Graduate School of Chinese Academy of Sciences, Beijing, 100000, P.R. China
3. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, 210008, P.R. China - ISSN：1608-3407
文摘
OsAMT is a high-affinity ammonium transporter responsible for NH 4 + uptake by rice plants. To investigate the expression patterns of OsAMT in different genotypes in relation to nitrogen accumulation, we measured the expression of OsAMT1.1, OsAMT1.2, and OsAMT1.3 using Real-Time PCR (RT-PCR) in GD (higher N accumulation) and NG (lower N accumulation) seedlings of the Oryza sativa L. cultivar treated with 0.1 mM NH4NO3 and 2 mM NH4NO3. We found that the expression level of OsAMT1.1 was significantly higher than those of OsAMT1.2 and OsAMT1.3 in the roots treated with 0.1 mM NH4NO3, suggesting that OsAMT1.1 contributed the most to N accumulation among the three genes. In GD root, OsAMT1.1 had significantly higher expression levels when it was up-regulated by 0.1 mM NH4NO3 than when down-regulated by 2 mM NH4NO3. OsAMT1.1 was mainly found in GD roots treated with 0.1 mM NH4NO3. We conclude that the OsAMT1.1 in GD roots, which was significantly up-regulated by low N and down-regulated by high N, was the dominating factor in determining the higher N acquisition in GD than in NG at 0.1 mM NH4NO3.