Optimized chromatographic separation and biological evaluation of 99m Tc-clarithromycin for infective inflammation diagnosis
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- 作者：E. H. Borai ; M. H. Sanad ; A. S. M. Fouzy
- 关键词：clarithromycin ; technetium ; 99m ; inflammation ; infection
- 刊名：Radiochemistry
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：58
- 期：1
- 页码：84-91
- 全文大小：309 KB
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M. H. Sanad (2)
A. S. M. Fouzy (3)
1. Analytical Chemistry Department, Hot Laboratories Center, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt
2. Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt
3. Food Toxin and Contaminants Department, National Research Centre, Tahrir Str. 33, Dokki, Cairo, Egypt - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Nuclear Chemistry
Chemistry
Russian Library of Science - 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
- ISSN：1608-3288
文摘
Clarithromycin antibiotic was labeled with technetium-99m by adding 99m Tc to clarithromycin in the presence of SnCl2·2H2O. The radiochemical yield of 99m Tc-clarithromycin was determined by chromatographic methods. Gel chromatographic method was investigated for separation of the reaction mixture using different eluents including NaCl and phosphate, citrate, and carbonate buffer solutions. Also, free 99m TcO 4 - and 99m Tc-clarithromycin were efficiently separated by reversed-phase HPLC on RP18 column. The maximum radiochemical yield reached 98 ± 0.2%. 99m Tc-clarithromycin is stable for 6 h. Biological distribution of 99m Tc-clarithromycin was studied for mice with the infection in the left thigh induced using Staphylococcus aureus. The target-to-nontarget (T/NT) ratio for 99m Tc-clarithromycin was found to be 7.33 ± 0.13 at 2 h after intravenous injection, with the subsequent gradual decline. This value is higher than that of commercially available 99m Tc-ciprofloxacin. The abscess to normal muscle ratio shows that 99m Tc-clarithromycin can be successfully used for infection imaging, allowing differentiation between bacterial infection and sterile inflammation.