Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA

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• 作者：Zhong-shan Gao ; Ying-tao Ma ; Xiang Zhou ; Zhao-wei Yang…
• 关键词：Lipid transfer protein ; Monoclonal antibodies ; Peach allergen ; Protein quantification ; Sandwich ELISA
• 刊名：Food Analytical Methods
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：9
• 期：4
• 页码：823-830
• 全文大小：668 KB
• 参考文献：Ahrazem O, Jimeno L, Lopez-Torrejon G, Herrero M, Espada JL, Sanchez-Monge R, Duffort O, Barber D, Salcedo G (2007) Assessing allergen levels in peach and nectarine cultivars. Ann Allergy Asthma Im 99:42–47CrossRef
  Asero R, Pravettoni V (2013) Anaphylaxis to plant-foods and pollen allergens with lipid transfer protein syndrome. Curr Opin Allergy Clin Immunol 13(4):379–385CrossRef
  Asero R, Mistrello G, Roncarolo D, de Vries SC, Gautier MF, Ciurana CL, Verbeek E, Mohammadi T, Knul-Brettlova V, Akkerdaas JH, Bulder I, Aalberse RC, van Ree R (2000) Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion. Int Arc Allergy Immunol 122:20–32CrossRef
  Asturias JA, Arilla MC, GomezBayon N, Martinez J, Martinez A, Palacios R (1997) Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen. J Allergy Clin Immun 100:365–372CrossRef
  Asturias JA, Arilla MC, Gomez-Bayon N, Aguirre M, Martinez A, Palacios R, Martinez J (1998) Cloning and immunological characterization of the allergen Hel a 2 (profilin) from sunflower pollen. Mol Immunol 35:469–478CrossRef
  Asturias JA, Arilla MC, Aguirre M, Gomez-Bayon N, Martinez A, Palacios R, Sanchez-Gascon F, Martinez J (1999) Quantification of profilins by a monoclonal antibody-based sandwich ELISA. J Immunol Methods 229:61–71CrossRef
  Ballmer-Weber BK (2002) Lipid transfer protein as a potential panallergen? Allergy 57:873–875CrossRef
  Breiteneder H, Ebner C (2000) Molecular and biochemical classification of plant-derived food allergens. J Allergy Clin Immun 106:27–36CrossRef
  Bublin M, Eiwegger T, Breiteneder H (2014) Do lipids influence the allergic sensitization process? J Allergy Clin Immunol 134(3):521–529CrossRef
  Burney PG, Potts J, Kummeling I, Mills EN, Clausen M, Dubakiene R, Barreales L, Fernandez-Perez C, Fernandez-Rivas M, Le TM, Knulst AC, Kowalski ML, Lidholm J, Ballmer-Weber BK, Braun-Fahlander C, Mustakov T, Kralimarkova T, Popov T, Sakellariou A, Papadopoulos NG, Versteeg SA, Zuidmeer L, Akkerdaas JH, Hoffmann-Sommergruber K, van Ree R (2014) The prevalence and distribution of food sensitization in European adults. Allergy 69(3):365–371CrossRef
  Carnes J, Fernandez-Caldas E, Gallego MT, Ferrer A, Cuesta-Herranz J (2002) Pru p 3 (LTP) content in peach extracts. Allergy 57:1071–1075CrossRef
  Chen L, Zhang SM, Illa E, Song LJ, Wu SD, Howad W, Arus P, van de Weg E, Chen KS, Gao ZS (2008) Genomic characterization of putative allergen genes in peach/almond and their synteny with apple. BMC Genomics 9:543CrossRef
  Diaz-Perales A, Lombardero M, Sanchez-Monge R, Garcia-Selles FJ, Pernas M, Fernandez-Rivas M, Barber D, Salcedo G (2000) Lipid-transfer proteins as potential plant panallergens: cross-reactivity among proteins of Artemisia pollen, Castanea nut and Rosaceae fruits, with different IgE-binding capacities. Clin Exp Allergy 30:1403–1410CrossRef
  Diaz-Perales A, Sanz ML, García-Casado G, Sánchez-Monge R, García-Selles FJ, Lombardero M, Polo F, Gamboa PM, Barber D, Salcedo G (2003) Recombinant Pru p 3 and natural Pru p 3, a major peach allergen, show equivalent immunologic reactivity: a new tool for the diagnosis of fruit allergy. J Allergy Clin Immunol 111(3):628–633CrossRef
  Duffort OA, Polo F, Lombardero M, Diaz-Perales A, Sanchez-Monge R, Garcia-Casado G, Salcedo G, Barber D (2002) Immunoassay to quantify the major peach allergen Pru p 3 in foodstuffs. Differential allergen release and stability under physiological conditions. J Agr Food Chem 50:7738–7741CrossRef
  Egger M, Hauser M, Mari A, Ferreira F, Gadermaier G (2010) The role of lipid transfer proteins in allergic diseases. Curr Allergy Asthma Rep 10(5):326–335CrossRef
  Fernandez-Rivas M, Gonzalez-Mancebo E, Rodriguez-Perez R, Benito C, Sanchez-Monge R, Salcedo G, Alonso D, Rosado A, Tejedor MA, Vila C, Casas ML (2003) Clinically relevant peach allergy is related to peach lipid transfer protein, pru p 3, in the Spanish population. J Allergy Clin Immunol 112:789–795CrossRef
  Gaier S, Marsh J, Oberhuber C, Rigby NM, Lovegrove A, Alessandri S, Briza P, Radauer C, Zuidmeer L, van Ree R, Hemmer W, Sancho AI, Mills C, Hoffmann-Sommergruber K, Shewry PR (2008) Purification and structural stability of the peach allergens Pru p 1 and Pru p 3. Mol Nutr Food Res 52:S220–S229
  Gao ZS, Gilissen LJW (2011) In: Jenks MA, Bebeli PJ (eds) Breeding of hypoallergenic fruits, in breeding for fruit quality, Firstth edn. Wiley, Hoboken, pp 105–126CrossRef
  Gao ZS, Yang ZW, Wu SD, Wang HY, Liu ML, Mao WL, Wang J, Gadermaier G, Ferreira F, Zheng M, van Ree R (2013) Peach allergy in China: a dominant role for mugwort pollen lipid transfer protein as a primary sensitizer. J Allergy Clin Immunol 131:224–226CrossRef
  Garino C, Zitelli F, Travaglia F, Coïsson JD, Cravotto G, Arlorio M (2012) Evaluation on the impact of sequential microwave/ultrasound processing on the IgE binding properties of Pru p 3 in treated peach juice. J Agr Food Chem 60:8755–8762CrossRef
  Greval RS, Mehra YN (1975) Preparation of allergenic extracts. Ind J Otolaryngol Head Neck Surg 27(2):59–61
  Kader JC (1996) Lipid-transfer proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 47:627–654CrossRef
  Kavaliunas A, Surkiene G, Dubakiene R, Stukas R, Zagminas K, Saulyte J, Burney PGJ, Kummeling I, Mills C (2012) EuroPrevall survey on prevalence and pattern of self-reported adverse reactions to food and food allergies among primary schoolchildren in Vilnius, Lithuania. Medicina-Lithuania 48(5):265–271
  Kirsch S, Fourdrilis S, Dobson R, Scippo ML, Maghuin-Rogister G, De Pauw E (2009) Quantitative methods for food allergens: a review. Anal Bioanal Chem 395:57–67CrossRef
  Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497CrossRef
  Li XW, Meng XQ, Jia HJ, Yu MY, Ma RJ, Wang LY, Cao K, Shen ZJ, Niu L, Tian JB, Chen MJ, Xie M, Arus P, Gao ZS, Aranzana MJ (2013) Peach genetic resources: diversity, population structure and linkage disequilibrium. BMC Genet 14:84CrossRef
  Li XW, Jiang J, Zhang LP, Yu Y, Ye ZW, Wang XM, Zhou JY, Chai ML, Zhang HQ, Arús P, Jia HJ, Gao ZS (2015) Identification of volatile and softening-related genes using digital gene expression profiles in melting peach. Tree Genet Genomes. doi:10.​1007/​s11295-015-0891-9
  Ma SK, Yin J, Jiang NN (2013) Component-resolved diagnosis of peach allergy and its relationship with prevalent allergenic pollens in China. J Allergy Clin Immunol 132:764–767CrossRef
  Ma YT, Zhou X, Gao ZS, Li XW, Jia HJ, Wu HX, Xie RJ, Wang ZQ, Yu ML, Cao K, Chen SJ, Li YH, Wang HY, van Ree R (2014) The PpLTP1 primary allergen gene is highly conserved in peach and has small variations in other Prunus species. Plant Mol Biol Rep 32:652–663CrossRef
  Pacios LF, Tordesillas L, Cuesta-Herranz J, Compes E, Sánchez-Monge R, Palacín A, Salcedo G, Diaz-Perales A (2008) Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: peach Pru p 3 allergen as a model. Mol Immunol 45(8):2269–2276CrossRef
  Pasquato N, Berni R, Folli C, Folloni S, Cianci M, Pantano S, Helliwell JR, Zanotti G (2006) Crystal structure of peach Pru p 3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens. J Mol Biol 356:684–694CrossRef
  Sancho AI, van Ree R, van Leeuwen A, Meulenbroek BJ, van de Weg EW, Gilissen LJWJ, Puehringer H, Laimer M, Martinelli A, Zaccharini M, Vazquez-Cortes S, Fernandez-Rivas M, Hoffmann-Sommergruber K, Mills ENC, Zuidmeer L (2008) Measurement of lipid transfer protein in 88 apple cultivars. Int Arch Allergy Imm 146:19–26CrossRef
  Toda M, Reese G, Gadermaier G, Schulten V, Lauer I, Egger M, Briza P, Randow S, Wolfheimer S, Kigongo V, Del Mar San Miguel Moncin M, Fötisch K, Bohle B, Vieths S, Scheurer S (2011) Protein unfolding strongly modulates the allergenicity and immunogenicity of Pru p 3, the major peach allergen. J Allergy Clin Immunol 128:1022–1030CrossRef
  Vassilopoulou E, Rigby N, Moreno FJ, Zuidmeer L, Akkerdaas J, Tassios I, Papadopoulos NG, Saxoni-Papageorgiou P, van Ree R, Mills C (2006) Effect of in vitro gastric and duodenal digestion on the allergenicity of grape lipid transfer protein. J Allregy Clin Immun 118(2):473–480CrossRef
  Yang ZW, Ma YT, Chen L, Xie RJ, Zhang B, Lu MD, Wu SD, Gilissen LJWJ, van Ree R, Gao ZS (2011) Differential transcript abundance and genotypic variation of four putative allergen-encoding gene families in melting peach. Tree Genet Genomes 7:903–916CrossRef
  Zuidmeer L, van Leeuwen WA, Budde IK, Cornelissen J, Bulder I, Rafalska I, Besoli NT, Akkerdaas JH, Asero R, Rivas MF, Mancebo EG, van Ree R (2005) Lipid transfer proteins from fruit: cloning, expression and quantification. Int Arch Allergy Imm 137:273–281CrossRef
  
• 作者单位：Zhong-shan Gao (1)
  Ying-tao Ma (1) (2)
  Xiang Zhou (1)
  Zhao-wei Yang (1)
  Hui-juan Jia (1)
  Ling Gao (1)
  Shan-dong Wu (1)
  Lu-yang Han (1)
  Xue-ying Yi (1)
  Hui-ying Wang (3)
  Jaap H. Akkerdaas (4)
  Ronald van Ree (5)
  
  1. Department of Horticulture/Allergy Research Center, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, College of Agriculture and Biotechnology, Zhejiang University, 310058, Hangzhou, China
  2. Life Science College, Luoyang Normal University, Luoyang, 471022, China
  3. Department of Allergy, the Second Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang, 310009, People’s Republic of China
  4. Department of Experimental Immunology, Academic Medical Center-University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands
  5. Department of Otorhinolaryngology and Experimental Immunology, Academic Medical Center-University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

Fruit is one of the most commonly reported food allergy sources in China, and peach lipid transfer protein (Pru p 3) has been identified as the major allergen inducing systematic symptoms. Crude allergen extracts and single component allergens have been used in food allergy diagnosis and immunotherapy. Reliable and sensitive analytical methods to quantify Pru p 3 content in fruit will help to identify low-allergenic cultivars among the abundant peach genetic resources in China. In this study, we developed a sensitive sandwich ELISA method to measure Pru p 3 in peach fruit based on two monoclonal antibodies with high sensitivity and linearity. Significant variability was observed in peach cultivars, with a much higher Pru p 3 level in peach peel than in pulp. This method will be very useful to select peach varieties with low Pru p 3 content and as a diagnostic product to detect hidden allergens in processed food.