Mass Spectrometric Confirmation of γ-Linolenic Acid Ester-Linked Ceramide 1 in the Epidermis of Borage Oil Fed Guinea Pigs

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• 作者：Kyong-Oh Shin ; Kunpyo Kim ; Sanghun Jeon ; Cho-Hee Seo ; Yong-Moon Lee ; Yunhi Cho
• 关键词：γ ; Linolenic acid ester ; linked ceramide 1 ; γ ; Linolenic acid ; Ceramide 1 ; LC–MS ; Borage oil ; Epidermis ; Guinea pigs
• 刊名：Lipids
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：50
• 期：10
• 页码：1051-1056
• 全文大小：666 KB
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• 作者单位：Kyong-Oh Shin (1)
  Kunpyo Kim (2)
  Sanghun Jeon (2)
  Cho-Hee Seo (1)
  Yong-Moon Lee (1)
  Yunhi Cho (2)
  
  1. College of Pharmacy, Chungbuk National University, Cheongju, Korea
  2. Department of Medical Nutrition, Graduate School of East–West Medical Science, Kyung Hee University, Yongin-Si, Gyeonggi-do, Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9307

文摘

Ceramide 1 (Cer1), a Cer species with eicosasphingenine (d20:1) amide-linked to two different ω-hydroxy fatty acids (C30wh:0:C32wh:1), which are, in turn, ester-linked to linoleic acid (LNA; 18:2n-6), plays a critical role in maintaining the structural integrity of the epidermal barrier. Prompted by the recovery of a disrupted epidermal barrier with dietary borage oil [BO: 36.5 % LNA and 23.5 % γ-linolenic acid (GLA; 18:3n-6)], in essential fatty acid (EFA)-deficient guinea pigs, we further investigated the effects of BO on the substitution of ester-linked GLA for LNA in these two epidermal Cer1 species by LC–MS in positive and negative modes. Dietary supplementation of BO for 2 weeks in EFA-deficient guinea pigs increased LNA ester-linked to C32wh:1/d20:1 and C30wh:0/d20:1 of Cer1. Moreover, GLA ester-linked to C32wh:1/d20:1, but not to C30wh:0/d20:1, of Cer1 was detected, which was further confirmed by the product ions of m/z 277.2 for ester-linked GLA and m/z 802.3 for the deprotonated C32wh:1/d20:1. C20-Metabolized fatty acids of LNA or GLA were not ester-linked to these Cer1 species. Dietary BO induced GLA ester-linked to C32wh:1/d20:1 of epidermal Cer1. Keywords γ-Linolenic acid ester-linked ceramide 1 γ-Linolenic acid Ceramide 1 LC–MS Borage oil Epidermis Guinea pigs