Duodenal–Jejunal Bypass Surgery Up-Regulates the Expression of the Hepatic Insulin Signaling Proteins and the Key Regulatory Enzymes of Intestinal Gluconeogenesis in Diabetic Goto–Kakizaki Rats

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• 作者：Dong Sun (1)
  Kexin Wang (1)
  Zhibo Yan (1)
  Guangyong Zhang (1)
  Shaozhuang Liu (1)
  Fengjun Liu (1)
  Chunxiao Hu (1)
  Sanyuan Hu (1)
  
• 关键词：Duodenal–jejunal bypass ; Bariatric surgery ; Gluconeogenesis ; Hepatic insulin signaling ; Goto–Kakizaki rat
• 刊名：Obesity Surgery
• 出版年：2013
• 出版时间：November 2013
• 年：2013
• 卷：23
• 期：11
• 页码：1734-1742
• 全文大小：
• 作者单位：Dong Sun (1)
  Kexin Wang (1)
  Zhibo Yan (1)
  Guangyong Zhang (1)
  Shaozhuang Liu (1)
  Fengjun Liu (1)
  Chunxiao Hu (1)
  Sanyuan Hu (1)
  
  1. Department of General Surgery, Qilu Hospital of Shandong University, #107 Wenhua Xi Road, Jinan, 250012, Shandong, People’s Republic of China
  
• ISSN：1708-0428

文摘

Background Duodenal–jejunal bypass (DJB), which is not routinely applied in metabolic surgery, is an effective surgical procedure in terms of type 2 diabetes mellitus resolution. However, the underlying mechanisms are still undefined. Our aim was to investigate the diabetic improvement by DJB and to explore the changes in hepatic insulin signaling proteins and regulatory enzymes of gluconeogenesis after DJB in a non-obese diabetic rat model. Methods Sixteen adult male Goto–Kakizaki rats were randomly divided into DJB and sham-operated groups. The body weight, food intake, hormone levels, and glucose metabolism were measured. The levels of protein expression and phosphorylation of insulin receptor-beta (IR-β) and insulin receptor substrate 2 (IRS-2) were evaluated in the liver. We also detected the expression of key regulatory enzymes of gluconeogenesis [phosphoenoylpyruvate carboxykinase-1 (PCK1), glucose-6-phosphatase-alpha (G6Pase-α)] in small intestine and liver. Results DJB induced significant diabetic improvement with higher postprandial glucagons-like peptide 1, peptide YY, and insulin levels, but without weight loss. The DJB group exhibited increased expression and phosphorylation of IR-β and IRS-2 in liver, up-regulated the expression of PCK1 and G6Pase-α in small intestine, and down-regulated the expression of these enzymes in liver. Conclusions DJB is effective in up-regulating the expression of the key proteins in the hepatic insulin signaling pathway and the key regulatory enzymes of intestinal gluconeogenesis and down-regulating the expression of the key regulatory enzymes of hepatic gluconeogenesis without weight loss. Our study helps to reveal the potential role of hepatic insulin signaling pathway and intestinal gluconeogenesis in ameliorating insulin resistance after metabolic surgery.