Seroprevalence and molecular detection of porcine sapovirus in symptomatic suckling piglets in Guangdong Province, China

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• 作者：Zi-Kui Liu (1) (2)
  Jian-Yong Li (3)
  Hu Pan (4)
  
• 关键词：Porcine sapovirus ; Prevalence ; Phylogenetic analysis ; Pig ; China
• 刊名：Tropical Animal Health and Production
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：46
• 期：3
• 页码：583-587
• 全文大小：193 KB
• 参考文献：1. Bank-Wolf, B.R., K?nig, M., Thiel, H.J., 2010. Zoonotic aspects of infections with noroviruses and sapoviruses. Veterinary Microbiology, 140, 204-12 CrossRef
  2. Barry, A.F., Alfieri, A.F., Alfieri, A.A., 2008. High genetic diversity in RdRp gene of Brazilian porcine sapovirus strains. Veterinary Microbiology, 131, 185-91. CrossRef
  3. Cunha, J.B., de Mendon?a, M.C., Miagostovich, M.P., Leite, J.P., 2010. Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil. Archives of Virology, 155, 1301-305. CrossRef
  4. Dufkova, L., Scigalkova, I., Moutelikova, R., Malenovska, H., Prodelalova, J., 2013. Genetic diversity of porcine sapoviruses, kobuviruses, and astroviruses in asymptomatic pigs: an emerging new sapovirus GIII genotype. Archives of Virology, 158, 549-58. CrossRef
  5. Farkas, T., Jiang, X., Guerrero, M.L., Zhong, W., Wilton, N., Berke, T., Matson, D.O., Pickering, L.K., Ruiz-Palacios, G., 2000. Prevalence and genetic diversity of human caliciviruses (HuCVs) in Mexican children. Journal of Medical Virology, 62, 217-23. CrossRef
  6. Farkas, T., Zhong, W.M., Jing, Y., Huang, P.W., Espinosa, S.M., Martinez, N., Morrow, A.L., Ruiz-Palacios, G.M., Pickering, L.K,, Jiang. X., 2004. Genetic diversity among sapoviruses. Archives of Virology, 2149, 1309-323.
  7. Green, K.Y., Ando, T., Balayan, M.S., Berke, T., Clarke, I.N., Estes, M.K., Matson, D.O., Nakata, S., Neill, J.D., Studdert, M.J., Thiel, H.J., 2000. Taxonomy of the caliciviruses. Journal of Infectious Diseases, 181, S322-30. CrossRef
  8. Guindon, S., Gascuel, O., 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696-04. CrossRef
  9. Guo, M., Hayes, J., Cho, K.O., Parwani, A.V., Lucas, L.M., Saif, L.J., 2001. Comparative pathogenesis of tissue culture-adapted and wild-type Cowden porcine enteric calicivirus (PEC) in gnotobiotic pigs and induction of diarrhea by intravenous inoculation of wild-type PEC. Journal of Virology, 75, 9239-251. CrossRef
  10. Jin, M., Yu. J.M., Li, H.Y., Zhang, Q., Cui, S.X., Tang, J.Y., Duan, Z.J., 2010. Genetic diversity of porcine sapoviruses from Lulong county in China. Bing Du Xue Bao, 26, 255-59. In Chinese.
  11. Johnsen, C.K., Midgley, S., B?ttiger, B., 2009. Genetic diversity of sapovirus infections in Danish children 2005-2007. Journal of Clinical Virology, 46, 265-69. CrossRef
  12. L’Homme, Y., Sansregret, R., Plante-Fortier, E., Lamontagne, A.M., Lacroix, G., Ouardani, M., Deschamps, J., Simard, G., Simard, C., 2009. Genetic diversity of porcine Norovirus and Sapovirus: Canada, 2005-2007. Archives of Virology, 154, 581-93. CrossRef
  13. Li, R.C., Liu, G.H., Huang, Z.B., Ding, J., Wang, Z.N., Xiao, C.T., Yu, X.L., 2012. Expression of VP1 gene and ELISA for detection of antibodies against porcine sapoviruses. Chinese Veterinary Science, 42, 5453-466 (in Chinese).
  14. Liu, G.H., Li, R.C., Li, J., Huang, Z.B., Xiao, C.T., Luo, W., Ge, M., Jiang, D.L., Yu, X.L., 2012a. Seroprevalence of porcine cytomegalovirus and sapovirus infection in pigs in Hunan province, China. Archives of Virology, 157, 521-24. CrossRef
  15. Liu, G.H., Li, R.C., Huang, Z.B., Yang, J., Xiao, C.T., Li, J., Li, M.X., Yan, Y.Q., Yu, X.L., 2012b. RT-PCR test for detecting porcine sapovirus in weanling piglets in Hunan Province, China. Tropical Animal Health and Production, 44, 1335-339. CrossRef
  16. Martella, V., Bányai, K., Lorusso, E., Bellacicco, A.L., Decaro, N., Mari, V., Saif, L., Costantini, V., De Grazia, S., Pezzotti, G., Lavazza, A., Buonavoglia, C., 2008. Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets. Virus Genes, 36, 365-73. CrossRef
  17. Martínez, M.A., Alcalá, A.C., Carruyo, G., Botero, L., Liprandi, F., Ludert, J.E., 2006. Molecular detection of porcine enteric caliciviruses in Venezuelan farms. Veterinary Microbiology, 116, 77-4. CrossRef
  18. Page, R.D., 1996. TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applied Bioscience, 12, 357-58.
  19. Phan, T.G., Trinh, Q.D., Yagyu, F., Okitsu, S., Ushijima, H., 2007. Emergence of rare sapovirus genotype among infants and children with acute gastroenteritis in Japan. European Journal of Clinical Microbiology & Infectious Diseases, 26, 21-7. CrossRef
  20. Posada, D., 2008. JModelTest phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253-256. CrossRef
  21. Reuter, G., Zimsek-Mijovski, J., Poljsak-Prijatelj, M., Di Bartolo, I., Ruggeri, F.M., Kantala, T., Maunula, L., Kiss, I., Kecskeméti, S., Halaihel, N., Buesa, J., Johnsen, C., Hjulsager, C.K., Larsen, L.E., Koopmans, M., B?ttiger, B., 2010. Incidence, diversity, and molecular epidemiology of sapoviruses in swine across Europe. Journal of Clinical Microbiology, 48, 363-68. CrossRef
  22. Rodríguez-Guillén, L., Vizzi, E., Alcalá, A.C., Pujol, F.H., Liprandi, F., Ludert, J.E., 2005. Calicivirus infection in human immunodeficiency virus seropositive children and adults. Journal of Clinical Microbiology, 33, 104-09.
  23. Saif, L.J., Bohl, E.H., Theil, K.W., Cross, R.F., House, J.A., 1980. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. Journal of Clinical Microbiology, 12, 105-11.
  24. Shen, Q., Zhang, W., Yang, S., Chen, Y., Ning, H., Shan, T., Liu, J., Yang, Z., Cui, L., Zhu, J., Hua, X., 2009. Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009. Archives of Virology, 154, 1625-630. CrossRef
  25. Shen, Q., Ren, R., Zhang, W., Yang, Z., Yang, S., Chen, Y., Cui, L., Hua, X., 2011. Prevalence of hepatitis E virus and porcine caliciviruses in pig farms of Guizhou province, China. Hepatits Monthly, 11, 459-63.
  26. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24, 4876-882. CrossRef
  27. Wang, Q.H., Souza, M., Funk, J.A., Zhang, W., Saif, L.J., 2006. Prevalence of noroviruses and sapoviruses in swine of various ages determined by reverse transcription-PCR and microwell hybridization assays. Journal of Clinical Microbiology, 44, 2057-062. CrossRef
  28. Yin, Y., Tohya, Y., Ogawa, Y., Numazawa, D., Kato, K., Akashi, H., 2006. Genetic analysis of calicivirus genomes detected in intestinal contents of piglets in Japan. Archives of Virology, 151, 1749-759. CrossRef
  
• 作者单位：Zi-Kui Liu (1) (2)
  Jian-Yong Li (3)
  Hu Pan (4)
  
  1. College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, People’s Republic of China
  2. Hunan Agricultural University Animal Pharmaceutical Co., Ltd., Liuyang, 410311, China
  3. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, 730050, China
  4. Lanzhou Institute of Animal Sciences and Pharmaceutics of CAAS, Lanzhou, 730050, China
  
• ISSN：1573-7438

文摘

The seroprevalence and genetic identification of sapovirus (SaV) in symptomatic suckling piglets were investigated in Guangdong Province, China, between November 2011 and April 2013. Serum (n--60) and diarrheic fecal (n--01) samples collected from symptomatic suckling piglets in Guangdong Province were evaluated for antibodies against SaV using indirect enzyme-linked immunosorbent assay (iELISA). The overall seroprevalence of SaV in symptomatic suckling piglets was 61.9?% (594/960). Positive animals were found in all regions with seroprevalence ranging from 52 to 67.8?%, but the difference was not statistically significant (P-gt;-.05). In addition, RNA of SaV was extracted from diarrheic fecal samples, and the partial polymerase gene was amplified by RT-PCR and then sequenced. Seven of 101 (6.9?%) samples were found to contain porcine SaV. Phylogenetic analysis showed that all the porcine SaV isolates belong to the porcine SaV genogroup III (GIII). This is the first report of SaV seroprevalence in symptomatic pigs in China.