Molecular phylogenetic studies based on rDNA ITS, cpDNA trnL intron sequence and cladode characteristics in nine Protasparagus taxa

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• 作者：Partha Sarathi Saha ; Sudipta Ray ; Mainak Sengupta ; Sumita Jha
• 关键词：Cladode ; cpDNA trnL intron ; Molecular phylogeny ; Protasparagus ; rDNA ITS
• 刊名：Protoplasma
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：252
• 期：4
• 页码：1121-1134
• 全文大小：1,692 KB
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• 作者单位：Partha Sarathi Saha (1)
  Sudipta Ray (1)
  Mainak Sengupta (2)
  Sumita Jha (1)
  
  1. Center of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India
  2. Department of Genetics, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Zoology
  
• 出版者：Springer Wien
• ISSN：1615-6102

文摘

The genus Asparagus comprises three subgenera of cladode bearing plants: Protasparagus, Asparagus, and Myrsiphyllum. The interspecific delimitation of the subgenus Protasparagus is ill-defined till date. In the present study, interspecific phylogenetic relationships among nine taxa of Protasparagus based on ribosomal DNA internal transcribed spacer region (ITS1-5.8S-ITS2) sequence and the chloroplast DNA trnL intron sequence conservation with their cladode morphology, anatomy, and stomatal characteristics have been analyzed for the first time. The monophyletic subgenus Protasparagus could be resolved into four strongly supported distinct subclades (I, II, III and IV) suggesting that the rDNA and cpDNA molecular phylogenies are explicitly congruent with the cladode characteristics of the subgenus Protasparagus. The present study also confirms the existing subgeneric classification of the genus Asparagus with the monophyletic origin of the dioecious subgenus Asparagus. The present work brings out phylogenetic and taxonomic relationships within the studied taxa of the subgenus Protasparagus therefore providing important background information for further studies on biogeography of a wide range of species.