In vivo behavior of NTBI revealed by automated quantification system

详细信息    查看全文

• 作者：Satoshi Ito ; Katsuya Ikuta ; Daisuke Kato ; Addo Lynda…
• 刊名：International Journal of Hematology
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：104
• 期：2
• 页码：175-181
• 全文大小：736 KB
• 刊物主题：Hematology; Oncology;
• 出版者：Springer Japan
• ISSN：1865-3774
• 卷排序：104

文摘

Non-Tf-bound iron (NTBI), which appears in serum in iron overload, is thought to contribute to organ damage; the monitoring of serum NTBI levels may therefore be clinically useful in iron-overloaded patients. However, NTBI quantification methods remain complex, limiting their use in clinical practice. To overcome the technical difficulties often encountered, we recently developed a novel automated NTBI quantification system capable of measuring large numbers of samples. In the present study, we investigated the in vivo behavior of NTBI in human and animal serum using this newly established automated system. Average NTBI in healthy volunteers was 0.44 ± 0.076 μM (median 0.45 μM, range 0.28–0.66 μM), with no significant difference between sexes. Additionally, serum NTBI rapidly increased after iron loading, followed by a sudden disappearance. NTBI levels also decreased in inflammation. The results indicate that NTBI is a unique marker of iron metabolism, unlike other markers of iron metabolism, such as serum ferritin. Our new automated NTBI quantification method may help to reveal the clinical significance of NTBI and contribute to our understanding of iron overload.KeywordsNon-transferrin bound iron (NTBI)Automated quantification systemIron overloadInflammation