Development of a new CMS system in pigeonpea utilizing crosses with Cajanus lanceolatus (WV Fitgz) van der Maesen

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• 作者：Sandhya Srikanth ; Rachit K. Saxena ; M. V. Rao ; Rajeev K. Varshney…
• 关键词：CMS ; C. lanceolatus ; Cytology ; Microtomy ; Meiosis ; Morphology ; Pollen fertility ; Sterility
• 刊名：Euphytica
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：204
• 期：2
• 页码：289-302
• 全文大小：2,382 KB
• 参考文献：Alexander MP (1969) Differential staining of aborted and nonaborted pollen. Stain Technol 44:117鈥?22PubMed
  Bohra A, Dubey A, Saxena RK, Penmetsa RV, Poornima KN, Kumar N, Farmer AD, Srivani G, Upadhyaya HD, Gothalwal R, Ramesh S, Singh D, Saxena K, Kishor PBK, Singh NK, Town CD, May GD, Cook DR, Varshney RK (2011) Analysis of BAC-end sequences (BESs) and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea (Cajanus spp.). BMC Plant Biol 11(1):15View Article
  Kaul CL, Singh SP (1969) Validity of stain tests in determining pollen viability of some papilionaceous plants. Indian J Agric Sci 39:1050鈥?055
  Kumar RV, Saxena KB (2013) Wild relatives of pigeonpea鈥攁n important resource for developing CMS systems. http://鈥媔crisat.鈥媋gropedia.鈥媔n/鈥媠ites/鈥媎efault/鈥媐iles/鈥媁ild relatives of Pigeonpea new (new).pdf. Accessed Oct 2013
  Malikarjuna N, Kalpana N (2004) Mechanisms of Cytoplasmic nuclear male sterility in pigeonpea wide cross C. cajan X C. acutifolius. Indian J Genet 64:115鈥?17
  Mallikarjuna N, Moss JP (1995) Production of hybrids between Cajanus platycarpus and Cajanus cajan. Euphytica 83(1):43鈥?6View Article
  Mallikarjuna N, Saxena KB (2005) A new cytoplasmic nuclear male-sterility system derived from cultivated pigeonpea cytoplasm. Euphytica 142:143鈥?48View Article
  Mallikarjuna N, Jadhav DR, Srikanth S, Saxena KB (2011) Cajanus platycarpus (Benth.) Maesen as the donor of new pigeonpea cytoplasmic male sterile (CMS) system. Euphytica 182:65鈥?1View Article
  Mallikarjuna N, Jadhav DR, Saxena KB, Srivastava RK (2012) Cytoplasmic male sterile systems in pigeonpea with special reference to A7 CMS. Electron J Plant Breed 3:983鈥?86
  O鈥橞rien TP, Feder N, Mc Cully ME (1964) Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma 59:368鈥?73View Article
  Pathak P, Sudi R, Wani SP, Sahrawat KL (2013) Hydrological behavior of Alfisols and Vertisols in the semi-arid zone: Implications for soil and water management. Agric Water Manage 118:12鈥?1View Article
  Reddy BVS, Green JM, Bisen SS (1978) Genetic male-sterility in pigeonpea. Crop Sci 18:362鈥?64View Article
  Saxena KB (2006) Seed production systems in pigeonpea. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, p 76. ISBN 92-9066-490-8
  Saxena KB (2013) A novel source of CMS in pigeonpea derived from Cajanus reticulatus. Indian J Genet Plant Breed 73:259鈥?63View Article
  Saxena KB, Singh L, Gupta MD (1990) Variation for natural out-crossing in pigeonpea. Euphytica 46:143鈥?48View Article
  Saxena KB, Sultana R, Mallikarjuna N, Saxena RK, Kumar RV, Sawargaonkar SL, Varshney RK (2010) Male-sterility systems in pigeonpea and their role in enhancing yield. Plant Breed 129:125鈥?34View Article
  Srikanth S, Rao MV, Mallikarjuna N (2013) Interspecific hybridization between Cajanus cajan (L.) Millsp. and C. lanceolatus (WV Fitgz) van der Maesen. Plant Genet Resour. doi:10.鈥?017/鈥婼147926211300036鈥?
  Virnich H (1967) Untersuchungen uber das Verhalten der mannlichen Sterilitat und anderer Eigenschaften bei polyploiden Zwiebeln (Allium cepa L.) als Grundlage fur eine Nutzung in der Hybrid. Auchtung Zeits Pflanzenzucht 58:205鈥?44
  
• 作者单位：Sandhya Srikanth (1) (2)
  Rachit K. Saxena (1)
  M. V. Rao (2)
  Rajeev K. Varshney (1)
  Nalini Mallikarjuna (1)
  
  1. Grain Legumes Program, International Crops Research Institute for Semi-Arid Tropics, Patancheru, 502 324, Andhra Pradesh, India
  2. Bharathidasan University, Tiruchchirappalli, 620 024, Tamil Nadu, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5060

文摘

Cytoplasmic male sterility is an important biological tool which is now available to pigeonpea breeders to exploit heterosis/hybrid vigor. A variety of CMS systems have been developed when wild relatives of pigeonpea from different gene pools were crossed as the female parent with cultivated types as the male parent. This paper reports a second source of CMS developed by using the cultivated pigeonpea as the female parent and one of its wild relative Cajanus lanceolatus (WV Fitgz) van der Maesen as the pollen donor, as such the A5 CMS system derived from C. acutifolius. All the F1 hybrids were evaluated to confirm hybridity using 27 simple sequence repeat (SSR) markers. SSR marker analysis of parents provided 17 polymorphic markers from a total of 27 SSR markers used. Subsequently polymorphic SSRs were used to confirm the hybridity of the F1 plants. F1 hybrid plants were crossed with a range of pigeonpea cultivars to identify maintainers of male sterility. Morphology of the F1 and backcross generations, cytology of the sterile as well as fertile floral buds derived from the crosses between sterile F1 hybrids and unrelated pigeonpea cultivars were studied. An important observation made was that male sterility was a post meiotic process. Microsporogenesis was normal until the tetrad stage, but none of them formed pollen grains. Instead, they grouped together within the pollen mother cell wall and the tetrads did not separate into individual pollen grains.