Optimization of industrial microorganisms: recent advances in synthetic dynamic regulators
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- 作者：Byung Eun Min ; Hyun Gyu Hwang ; Hyun Gyu Lim…
- 关键词：Dynamic regulator ; Synthetic biology ; Industrial microorganism ; Quorum sensing ; Transcription factors
- 刊名：Journal of Industrial Microbiology & Biotechnology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：44
- 期：1
- 页码：89-98
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Biochemistry, general; Inorganic Chemistry; Genetic Engineering; Biotechnology; Bioinformatics;
- 出版者：Springer Berlin Heidelberg
- ISSN：1476-5535
- 卷排序：44
文摘
Production of biochemicals by industrial fermentation using microorganisms requires maintaining cellular production capacity, because maximal productivity is economically important. High-productivity microbial strains can be developed using static engineering, but these may not maintain maximal productivity throughout the culture period as culture conditions and cell states change dynamically. Additionally, economic reasons limit heterologous protein expression using inducible promoters to prevent metabolic burden for commodity chemical and biofuel production. Recently, synthetic and systems biology has been used to design genetic circuits, precisely controlling gene expression or influencing genetic behavior toward a desired phenotype. Development of dynamic regulators can maintain cellular phenotype in a maximum production state in response to factors including cell concentration, oxygen, temperature, pH, and metabolites. Herein, we introduce dynamic regulators of industrial microorganism optimization and discuss metabolic flux fine control by dynamic regulators in response to metabolites or extracellular stimuli, robust production systems, and auto-induction systems using quorum sensing.