Chiral separation of ketoprofen on an achiral NH₂ column by HPLC using vancomycin as chiral mobile phase additive

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• 作者：Dehbiya Gherdaoui ; Hafsa Bekdouche ; Said Zerkout…
• 关键词：Ketoprofen ; Chiral separation ; Vancomycin ; Chiral mobile phase additive
• 刊名：Journal of the Iranian Chemical Society
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：13
• 期：12
• 页码：2319-2323
• 全文大小：537 KB
• 刊物主题：Analytical Chemistry; Inorganic Chemistry; Physical Chemistry; Biochemistry, general; Organic Chemistry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1735-2428
• 卷排序：13

文摘

A high-performance liquid chromatographic method for chiral separation of ketoprofen racemate was developed. (R)- and (S)-ketoprofen enantiomers were separated on a LiChrosorb NH₂ column (250 mm × 4.6 mm, i.d 5 µm) at 20 °C, using 2-propanol/potassium dihydrogen phosphate buffer (pH 6.0, 0.05 M) (50:50 v/v). Containing vancomycin as the mobile phase, at a flow rate of 0.8 ml min−1 and detection wavelength of UV, the detector was set at 310 nm. Under these conditions, ketoprofen enantiomers could be separated with a selectivity factor (α) of 2.172 and a resolution (Rs) of 4.78 using extremely low concentrations of the vancomycin chiral additive.