A simple screening method for analysis of cell adhesive strength cultured on zein microspheres

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• 作者：Hui Yang 杨 辉 ; Weiying Li 李伟迎 ; Jinye Wang 王瑾晔
• 关键词：Key wordszein microcarrier ; surface topography ; cell adhesion ; beating experiment
• 刊名：Journal of Shanghai Jiaotong University (Science)
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：22
• 期：1
• 页码：10-14
• 全文大小：
• 刊物类别：Engineering
• 刊物主题：Electrical Engineering; Materials Science, general; Computer Science, general; Architecture, general; Life Sciences, general;
• 出版者：Shanghai Jiaotong University Press
• ISSN：1995-8188
• 卷排序：22

文摘

Cell adhesion plays an important role in the microcarrier culture. Therefore, it is necessary to find a simple method to evaluate the cell adhesive strength. By the glycerol method, we prepared zein microcarriers. Both microcarrier surface morphology and cells on the microcarriers could be observed clearly when the microcarriers and the cells were stained by acridine orange (AO). Pre-treatment of soaking and sterilizing processes was necessary for cell culture, but it did not alter the morphology of zein microcarriers. Three kinds of cells were cultured on zein microcarriers for 24 h. Vero cells showed the strongest adhesion, while L929 cells were easily detached from the microcarriers after a simple beating operation. The proliferation curve of Vero cells cultured on the microcarriers displayed no difference with that on Cytodex 1.