Development of Sandwich ELISA and Immunochromatographic Strip for the Detection of Peanut Allergen Ara h 2

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• 作者：Juan Peng ; Shanshan Song ; Liqiang Liu ; Hua Kuang ; Chuanlai Xu
• 关键词：Peanut allergen ; Ara h 2 ; Monoclonal antibody ; Sandwich ELISA ; Immunochromatographic strip
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：8
• 期：10
• 页码：2605-2611
• 全文大小：465 KB
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• 作者单位：Juan Peng (1)
  Shanshan Song (1)
  Liqiang Liu (1)
  Hua Kuang (1)
  Chuanlai Xu (1)
  
  1. State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, People鈥檚 Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

In order to develop highly sensitive immunoassays for detecting the major peanut allergen, Ara h 2, we prepared six monoclonal antibodies (mAb) with high affinity to Ara h 2. Using mAb 5 as a capture antibody and HRP-labeled mAb 1 as a detection antibody, a sandwich enzyme-linked immunoassay (ELISA) was established with a limit of detection (LOD) of 0.02 ng/mL. An immunochromatographic strip based on these two mAbs was also established with a LOD of 1 ng/mL. Both tests showed high specificity in cross-reactivity experiments. Sample analyses indicated that these two immunoassays were feasible, accurate, and highly sensitive for measuring allergen Ara h 2 of food. Keywords Peanut allergen Ara h 2 Monoclonal antibody Sandwich ELISA Immunochromatographic strip