Effects of Compound Shenhua Tablet ( on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury

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• 作者：Yue Yang (1) (2)
  Ri-bao Wei (1)
  Xiao-yong Zheng (1)
  Qiang Qiu (1)
  Shao-yuan Cui (1)
  Zhong Yin (1)
  Suo-zhu Shi (1)
  Xiang-mei Chen (1)
  
• 关键词：Compound Shenhua Tablet ; astragaloside ; renal ischemic reperfusion injury ; Na+ ; K+ ; adenosinetriphosphatase
• 刊名：Chinese Journal of Integrative Medicine
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：20
• 期：3
• 页码：200-208
• 全文大小：5,553 KB
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• 作者单位：Yue Yang (1) (2)
  Ri-bao Wei (1)
  Xiao-yong Zheng (1)
  Qiang Qiu (1)
  Shao-yuan Cui (1)
  Zhong Yin (1)
  Suo-zhu Shi (1)
  Xiang-mei Chen (1)
  
  1. State Discipline and State Key Laboratory of Kidney Disease (Chinese PLA General Hospital, 2011DAV00088), Beijing, 100853, China
  2. Medical School of Nankai University, Tianjin, 300071, China
  
• ISSN：1993-0402

文摘

Objective To observe the effect of Compound Shenhua Tablet ( SHT) on the sodiumpotassium- exchanging adenosinetriphosphatase (Na+-K+-ATPase) in the renal tubular epithelial cells of rats with acute ischemic reperfusion and to investigate the mechanisms underlying the effects of SHT on renal ischemic reperfusion injury (RIRI). Methods Fifty male Wistar rats were randomly divided into the sham surgery group, model group, astragaloside group [150 mg/(kg·d)], SHT low-dose group [1.5 g/(kg·d)] and SHT high-dose group [3.0 g/(kg·d)], with 10 rats in each group. After 1 week of continuous intragastric drug administration, surgery was performed to establish the model. At either 24 or 72 h after the surgery, 5 rats in each group were sacrificed, blood biochemistry, renal pathology, immunoblot and immunohistochemical examinations were performed, and double immunofluorescence staining was observed under a laser confocal microscope. Results Compared with the sham surgery group, the serum creatinine (SCr) and blood urea nitrogen (BUN) levels were significantly increased, Na+-K+-ATPase protein level was decreased, and kidney injury molecule-1 (KIM-1) protein level was increased in the model group after the surgery (P<0.01 or P<0.05). Compared with the model group, the SCr, BUN, pathological scores, Na+-K+-ATPase, and the KIM-1 protein level of the three treatment groups were significantly improved at 72 h after the surgery (P<0.05 or P<0.01). And the SCr, BUN of the SHT low- and high-dose groups, and the pathological scores of the SHT high-dose group were significantly lower than those of the astragaloside group (P<0.05). The localizations of Na+-K+-ATPase and megalin of the model group were disrupted, with the distribution areas overlapping with each other and alternately arranged. The severity of the disruption was slightly milder in three treatment groups compared with that of the model group. The results of immunofluorescence staining showed that the SHT high-dose group had a superior effect as compared with the astragaloside group and the SHT low-dose group. Conclusions The SHT effectively alleviated RIRI caused by ischemic reperfusion, promoted the recovery of the polarity of renal tubular epithelial cells, and protected the renal tubules. The therapeutic effects of SHT were superior to those of astragaloside as a single agent.