Effects of monoclonal antibodies against human stathmin 1 combined paclitaxel on proliferation of human hepatocellular carcinoma cell lines

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• 作者：Shuang Wang (1)
  Xiaoli Liu (1)
  Shaofei Yuan (2)
  Wenjun Chen (2)
  Senming Wang (3)
  Na Xu (1)
  
• 关键词：hepatocellular carcinoma ; monoclonal antibodies against stathmin 1 ; paclitaxel ; proliferation ; apoptosis
• 刊名：The Chinese-German Journal of Clinical Oncology
• 出版年：2009
• 出版时间：October 2009
• 年：2009
• 卷：8
• 期：10
• 页码：603-606
• 全文大小：303KB
• 参考文献：1. Tang ZY. Diagnosis and treatment of hepatocellular carcinoma-current status and prospects. Chin J Bases Clin General Surg (Chinese), 2003, 10: 191.
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• 作者单位：Shuang Wang (1)
  Xiaoli Liu (1)
  Shaofei Yuan (2)
  Wenjun Chen (2)
  Senming Wang (3)
  Na Xu (1)
  
  1. Department of Haematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China
  2. Department of Oncology, Ruian People鈥檚 Hospital, Wenzhou Medical College, Ruian, 325200, China
  3. Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China
  

文摘

Objective We investigated the effects of monoclonal antibodies against stathmin 1 combined paclitaxel on the proliferation of HCC cells. Methods HepG2 cells were treated with monoclonal antibodies against stathmin 1, paclitaxel alone or their combination, with the untreated cells used as the control, 24, 48, 72, 96 h later, the cell growth condition was observed by invert microscope and inhabitation rate was studied by MTT assay; The apoptosis was analyzed by flow cytometry with Annexin V/PI. Results The population decreased and shape, size changed after treating with different concentration of experimental groups. Monoclonal antibodies against stathmin 1 and paclitaxel used alone or in combination both inhibited the proliferation of HepG2 cells, the inhibition ratio of their combination was more higher (P < 0.05), and a synergistic effect of the two agents was noted in their combined action (P < 0.05). Combined treatment of the cells resulted in significantly higher apoptosis rate than that in the other groups (P < 0.05). Conclusion Monoclonal antibodies against stathmin 1 and paclitaxel used alone or in combination both can inhibit proliferation of HepG2 cells and induce apoptosis. A synergistic effect is observed between the monoclonal antibodies against stathmin 1 and paclitaxel in their inhibition of HepG2 cell proliferation.