文摘
Supercritical fluid extraction (SFE) was utilized for the first time to extract phenolics in date seeds. Orthogonal array design (OA9 (34)) was applied to optimize extraction variables including extraction temperature, extraction pressure, extraction time, and number of extraction. Optimum values of extraction variables were analyzed and number of extractions was found to have a significant effect on total phenolics content (TPC). Optimal SFE conditions for maximum yield of TPC were 50聽掳C, 350聽bar, and two repeated extractions, each for 2聽h. Under optimal condition, the TPC was increased to 441.57聽mg gallic acid equivalents/100聽g fresh weight. Several phenolic compounds were detected in date seeds including chlorogenic acid, rutin, ellagic acid, quercetin, and two unidentified compounds of phenolic acids. In addition, phenolics of date seeds showed a higher antioxidant activity than ascorbic acid at the same concentration in the range of 1.0鈥?.0聽mg/L.