Isolation and characterization of fifteen polymorphic tetranucleotide microsatellites for Ichthyophis bannanicus (Gymnophiona: Ichthyophiidae)

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• 作者：Guifen Li (1)
  Xia Luo (2)
  Hui Wang (2)
  Yongjian Bei (1)
  Wenbo Shi (2)
  Shaoquan Meng (1)
  Baowei Zhang (2)
  
• 关键词：Ichthyophis bannanicus ; Microsatellites ; PCR primers ; Polymorphism
• 刊名：Conservation Genetics Resources
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：2
• 期：1-supp
• 页码：129-131
• 全文大小：156KB
• 参考文献：1. Fei L, Hu S, Ye C, Huang Y (2006) Fauna Sinica amphibia. Science Press, Beijing
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• 作者单位：Guifen Li (1)
  Xia Luo (2)
  Hui Wang (2)
  Yongjian Bei (1)
  Wenbo Shi (2)
  Shaoquan Meng (1)
  Baowei Zhang (2)
  
  1. Department of Chemistry & Life Science, Yulin Normal University, Yulin, 537000, China
  2. School of Life Science, Anhui University, Hefei, 230039, China
  

文摘

We characterize fifteen polymorphic microsatellite loci isolated from Ichthyophis bannanicus genomic libraries, which enriched for ATAG-motif microsatellites. The fifteen Loci were screened on a group of 32 individuals from Mengla population. These markers revealed a relatively high degree of genetic diversity (3-3 alleles per locus) and heterozygosity (Ho ranged from 0.036 to 0.935 and He ranged from 0.105 to 0.892). In the fifteen loci, just three showed deviation from Hardy–Weinberg equilibrium (HWE). The high level of polymorphism revealed by these microsatellite markers will prove useful for the study of gene flow, population structure and evolutionary history of Ichthyophis bannanicus.