Distribution of Metabolically Active Prokaryotes (Archaea and Bacteria) throughout the Profiles of Chernozem and Brown Semidesert Soil

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• 作者：M. V. Semenov ; N. A. Manucharova ; A. L. Stepanov
• 关键词：method of fluorescent in situ hybridization (FISH) ; 16S rRNA ; soil microbial community ; bacteria ; archaea ; Calcisols
• 刊名：Eurasian Soil Science
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：49
• 期：2
• 页码：217-225
• 全文大小：757 KB
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• 作者单位：M. V. Semenov (1) (2)
  N. A. Manucharova (1)
  A. L. Stepanov (1)
  
  1. Faculty of Soil Science, Moscow State University, Leninskie gory, 119991, Moscow, Russia
  2. Dokuchaev Soil Science Institute, per. Pyzhevskii 7, Moscow, 119017, Russia
  
• 刊物主题：Geotechnical Engineering & Applied Earth Sciences;
• 出版者：Springer US
• ISSN：1556-195X

文摘

The distribution of metabolically active cells of archaea and bacteria in the profiles of typical chernozems (Voronezh oblast) and brown semidesert soils (Astrakhan oblast) of natural and agricultural ecosystems was studied using the method of fluorescent in situ hybridization (FISH). The studied soils differed sharply in the microbial biomass and in the numbers of metabolically active cells of archaea and bacteria. The number of active bacterial cells was 3.5–7.0 times greater than that of archaea. In the arable chernozem, the numbers of active cells of archaea and bacteria were 2.6 and 1.5 times, respectively, lower than those in the chernozem under the shelterbelt. The agricultural use of the brown semidesert soil had little effect on the abundances of bacteria and archaea. The soil organic carbon content was the major factor controlling the numbers of metabolically active cells of both domains. However, the dependence of the abundance of bacteria on the organic matter content was more pronounced. The decrease in the organic carbon and total nitrogen contents down the soil profiles was accompanied by the decrease in the bacteria: archaea ratio attesting to a better adaptation of archaea to the permanent deficiency of carbon and nitrogen. The bacteria: archaea ratio can serve as an ecotrophic indicator of the state of soil microbial communities.