Evidence of a new Tomato yellow leaf curl virus in Japan and its detection using PCR

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• 作者：Shigenori Ueda ; Shigeharu Takeuchi ; Mie Okabayashi ; Kaoru Hanada ; Kenta Tomimura and Toru Iwanami
• 关键词：Geminivirus ; Begomovirus ; Tomato yellow leaf curl virus ; Diagnosis
• 刊名：Journal of General Plant Pathology
• 出版年：2005
• 出版时间：August 2005
• 年：2005
• 卷：71
• 期：4
• 页码：319-325
• 全文大小：370 KB

文摘

A new isolate of Tomato yellow leaf curl virus (TYLCV) has been identified from tomato plants in Kochi Prefecture in Japan and designated TYLCV-[Tosa]. The complete nucleotide sequence of the isolate was determined and found to consist of 2781 nt. In phylogenetic analyses of entire nucleotide sequences, TYLCV-[Tosa] was delineated as a single branch and was more closely related to TYLCV-[Almeria] than TYLCV isolates Ng, Sz, or Ai reported in Japan, which had spread since 1996. Isolate TYLCV-[Tosa] is suggested to be a newly introduced, novel isolate of TYLCV that dispersed into Kochi Prefecture. In addition, a rapid method using the polymerase chain reaction to separate TYLCV isolates into four genetic groups was established. This method would be useful for reliable diagnosis based on genetic differences among isolates of TYLCV.