Highly sensitive electrochemiluminescence biosensors for cholesterol detection based on mesoporous magnetic core–shell microspheres
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- 作者：Juanjuan Zhang (1)
Shihong Chen (1)
Xingrong Tan (2)
Xia Zhong (1)
Dehua Yuan (1)
Yinfeng Cheng (1) - 关键词：Biosensor ; Cholesterol oxidase ; Electrochemiluminescence ; Luminol ; Magnetic core–shell microspheres
- 刊名：Biotechnology Letters
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：36
- 期：9
- 页码：1835-1841
- 全文大小：672 KB
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Shihong Chen (1)
Xingrong Tan (2)
Xia Zhong (1)
Dehua Yuan (1)
Yinfeng Cheng (1)
1. Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People’s Republic of China
2. Department of Endocrinology, 9th People’s Hospital of Chongqing, Chongqing, 400700, People’s Republic of China - ISSN：1573-6776
文摘
A sensitive electrochemiluminescence (ECL) biosensor for cholesterol detection based on multifunctional core–shell structured microspheres (Fe3O4@SiO2–Au@mpSiO2) is reported. This microsphere consisted of a core of silica-coated magnetite nanoparticle, an active transition layer of gold nanoparticles and a mesoporous silica shell. Scanning electron microscopy was employed to observe the morphology of the nanomaterials and transmission electron microscopy was used to further confirm the subtle structure of Fe3O4@SiO2–Au@mpSiO2. The microspheres possessed a large surface area that increased enzyme loading, and an active transition layer gold nanoparticles enhanced the ECL signal. They were used to immobilize cholesterol oxidase for cholesterol detection with a high sensitivity, low detection limit and wide linear range. The linear range was from 0.83 to 2.62?mM with a detection limit of 0.28?μM (S/N?=?3). Moreover, the reproducibility, stability and selectivity of the biosensor were established.