A biorecognition system for concanavalin a using a glassy carbon electrode modified with silver nanoparticles, dextran and glucose oxidase

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• 作者：Xiaofang Liu ; Xin Ou ; Qiyi Lu ; Shihong Chen ; Shaping Wei
• 关键词：Concanavalin A ; Bio ; recognition system ; Silver nanoparticles ; Reduced graphene oxide ; Biosensor
• 刊名：Microchimica Acta
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：182
• 期：3-4
• 页码：797-803
• 全文大小：606 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

Concanavalin A (Con A) was chosen as a model lectin protein for an electrochemical study on the interaction between lectins and carbohydrates. A composite prepared from reduced graphene oxide and silver nanoparticles (rGO-AgNPs) acted as a redox probe, and phenoxy-derivatized dextran (DexP) and glucose oxidase (GOx) acted as recognition elements. The rGO-AgNPs, DexP and GOx were deposited on the surface of a glassy carbon electrode. The immobilized DexP and GOx showed high affinity for the target lectin Con A. The surface-modified biosensor was characterized by Fourier transform infrared?spectroscopy, scanning electron microscopy, UV–vis absorption spectra, cyclic voltammetry and electrochemical impedance spectroscopy. Due to the high affinity of Con A for both DexP and GOx, the biosensor displays a detection limit for Con A that is as low as 0.67?ng?mL? at a working voltage of 48?mV. The response is linear in the 2.0-22?ng?mL-1 concentration range, thus offering a sensitive means for the determination of Con A. Graphical Abstract This work presented a simple bio-recognition system with rGO-AgNPs as redox probes, DexP and GOx as bio-recognition elements for sensitive detection of Con A. The detection limit for Con A that is as low as 0.67?ng?mL? at a working voltage of 48?mV.