The aflatoxin-detoxifizyme specific expression in mouse parotid gland

详细信息    查看全文

• 作者：Li-zeng Guan ; Yu-ping Sun ; Jin-shun Cai ; Han-dong Wu ; Long-zheng Yu…
• 关键词：Parotid gland ; Aflatoxin ; detoxifizyme ; Transgenic mice
• 刊名：Transgenic Research
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：24
• 期：3
• 页码：489-496
• 全文大小：522 KB
• 参考文献：Alberts J, Engelbrecht Y, Steyn P, Holzapfel W, Van Zyl W (2006) Biological degradation of aflatoxin B1 Rhodococcus erythropolis cultures. Int J Food Microbiol 109:121-26View Article PubMed
  Alien ND, Cran DG, Barton SC, Hettle S, Reik W, Surani MA (1988) Transgenes as probes for active chromosomal domains in mouse development. Nature 333:852-55View Article
  Alshawi R, Kinnaird J, Burke J, Bishop JO (1990) Expression of a foreign gene in a line of transgenic mice is modulated by a chromosomal position effect. Mol Cell Biol 10:1192-198
  Aravind K, Patil V, Devegowda G, Umakantha B, Ganpule S (2003) Efficacy of esterified glucomannan to counteract mycotoxicosis in naturally contaminated feed on performance and serum biochemical and hematological parameters in broilers. Poult Sci 82:571-76View Article PubMed
  Basmacioglu H, Oguz H, Ergul M, Col R, Birdane Y (2005) Effect of dietary esterified glucomannan on performance, serum biochemistry and haematology in broilers exposed to aflatoxin. Czech J Anim Sci 50:31-9
  Bassir O, Emafo PO (1970) Oxidative metabolism of aflatoxin B1 by mammalian liver slices and microsomes. Biochem Pharmacol 19:1681-687View Article PubMed
  Bennett JW, Goldblatt L (1973) The isolation of mutants of Aspergillus flavus and A. parasiticus with altered aflatoxin producing ability. Med Mycol 11:235-41View Article
  Chen B, Wang Y, Yu H, Xu Q (2011) Effects of aflatoxin-detoxifizyme on growth performance and liver biochemical indices of broilers fed with aflatoxin B1. Front Agric China 5:594-97View Article
  Denissenko MF, Cahill J, Koudriakova TB, Gerber N, Pfeifer GP (1999) Quantitation and mapping of aflatoxin B1-induced DNA damage in genomic DNA using aflatoxin B1-8, 9-epoxide and microsomal activation systems. Mutat Res, Fundam Mol Mech Mutagen 425:205-11View Article
  Eaton DL, Gallagher EP (1994) Mechanisms of aflatoxin carcinogenesis. Annu Rev Pharmacol Toxicol 34:135-72View Article PubMed
  Golovan SP, Hayes MA, Phillips JP, Forsberg CW (2001a) Transgenic mice expressing bacterial phytase as a model for phosphorus pollution control. Nat Biotechnol 19:429-33View Article PubMed
  Golovan SP, Meidinger RG, Ajakaiye A, Cottrill M, Wiederkehr MZ, Barney DJ, Plante C, Pollard JW, Fan MZ, Hayes MA (2001b) Pigs expressing salivary phytase produce low-phosphorus manure. Nat Biotechnol 19:741-45View Article PubMed
  Guan LZ, Sun YP, Xi QY, Wang JL, Zhou JY, Shu G, Jiang QY, Zhang YL (2013) β-Glucanase specific expression in the parotid gland of transgenic mice. Transgenic Res 22:805-12View Article PubMed
  Han XY, Huang QC, Li WF, Jiang JF, Xu ZR (2008) Changes in growth performance, digestive enzyme activities and nutrient digestibility of cherry valley ducks in response to aflatoxin B1 levels. Livest Sci 119:216-20View Article
  Hogan B, Costantini F, Lacy E (1986) Manipulating the mouse embryo: a laboratory manual. Cold spring harbor laboratory Cold Spring Harbor, NY
  Liu DL, Yao DS, Liang R, Ma L, Cheng WQ, Gu LQ (1998) Detoxification of Aflatoxin B1 by Enzymes Isolated from Armillariella tabescens. Food Chem Toxicol 36:563-74View Article PubMed
  Liu DL, Yao DS, Liang YQ, Zhou TH, Song YP, Zhao L, Ma L (2001) Production, purification, and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20). Food Chem Toxicol 39:461-66View Article PubMed
  Massey TE, Stewart RK, Daniels JM, Liu L (1995) Biochemical and molecular aspects of mammalian susceptibility to aflatoxin B1 carcinogenicity. Exp Biol Med 208:213-27View Article
  Mercado C, Real M, Rosario R (1991) Chemical detoxification of aflatoxin-containing copra. J Food Sci 56:733-35View Article
  Natarajan K (1991) Chemical inactivation of aflatoxins in peanut protein ingredients. J Environ Pathol Toxicol Oncol: Off. Organ Int Soc Environ Toxicol Cancer 11:217-27
  Oguz H, Kurtoglu V (2000) Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis. Br Poult Sci 41:512-17View Article PubMed
  Ortatatli M, O?uz H (2001) Ameliorative effects of dietary clinoptilolite on pathological changes in broiler chickens during aflatoxicosis. Res Vet Sci 71:59-6View Article PubMed
  Park DL, Liang B (1993) Perspectives on aflatoxin control for human food and animal feed. Trends Food Sci Technol 4:334-42View Article
  Patterson D (1973) Metabolism as a factor in determining the toxic action of the aflatoxins in different animal species. Food Cosmet Toxicol 11:287-94View Article PubMed
  Payne G, Brown M (1998) Genetics and physiology of aflatoxin biosynthesis. Annu Rev Phytopathol 36:329-62View Article PubMed
  Robens J, Richard J (1992) Aflatoxins in animal and human health. Rev Environ Contam Toxicol 127:69-4
  Rustom I (1997) Aflatoxin in food and feed: occurrence, legislation and inactivation by physical methods. Food Chem 59:57-7View Article
  Samarajeewa U, Sen A, Fernando S, Ahmed E, Wei C (
• 作者单位：Li-zeng Guan (1)
  Yu-ping Sun (2)
  Jin-shun Cai (1)
  Han-dong Wu (3)
  Long-zheng Yu (1)
  Yong-liang Zhang (4)
  Qian-yun Xi (4)
  
  1. Agriculture College, Yanbian University, Gongyuan Road, Yanji, 133000, China
  2. Institute of Animal Science, Guangdong Academy of Agriculture Science, Guangzhou, 510640, China
  3. Liaoning Medical University, Jinzhou, 121001, China
  4. College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, Guangzhou, 510642, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Molecular Medicine
  Plant Genetics and Genomics
  Animal Genetics and Genomics
  Plant Sciences
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-9368

文摘

The aflatoxin-detoxifizyme (ADTZ) gene derived from Armillariella tabescens was cloned into parotid gland-specific expression vector (pPSPBGPneo) to construct the parotid gland-specific vector expressing ADTZ (pPSPBGPneo-ADTZ). Transgenic mice were generated by microinjection and identified by using PCR and Southern blotting analysis. PCR and Southern blotting analysis showed that total six transgenic mice carried the ADTZ gene were generated. RT-PCR analysis indicated that the expression of ADTZ mRNA could be detected only in parotid glands of the transgenic mice. The ADTZ activity in the saliva was found to be 3.72?±?1.64?U/mL. After feeding a diet containing aflatoxin B1 (AFB1) for 14 days, the effect of ADTZ on serum biochemical indexes and AFB1 residues in serum and liver of mice were evaluated. The results showed that total protein and globulin contents in the test treatment (transgenic mice) produced ADTZ were significantly higher than that of the positive control, while alanine aminotransferase and aspartate aminotransferase activity in serum of the test treatment (transgenic mice) were remarkably lower compared to that of the positive control (P?<?0.05). Moreover, AFB1 residues in serum and liver of the test treatment (transgenic mice) were significantly lower compared with that of the positive control (P?<?0.05). These results in the study confirmed that ADTZ produced in transgenic mice could reduce, even eliminate the negative effects of AFB1 on mice.