ILEI drives epithelial to mesenchymal transition and metastatic progression in the lung cancer cell line A549

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• 作者：Qi Song (1)
  Wei Sheng (2)
  Xiaomei Zhang (3)
  Shunchang Jiao (1)
  Fang Li (1)
  
• 关键词：ILEI ; Lung cancer ; Metastasis ; Epithelial–mesenchymal transition ; Cancer stem cells
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：35
• 期：2
• 页码：1377-1382
• 全文大小：312 KB
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• 作者单位：Qi Song (1)
  Wei Sheng (2)
  Xiaomei Zhang (3)
  Shunchang Jiao (1)
  Fang Li (1)
  
  1. Department of Oncology, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China
  2. Department of Cardiovascular Surgery, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China
  3. Department of Gastroenterology, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China
  
• ISSN：1423-0380

文摘

Transforming growth factor beta (TGF-β) induces epithelial–mesenchymal transition (EMT) accompanied by cellular differentiation and migration. Despite extensive transcriptomic profiling, identification of TGF-β-inducible, EMT-specific genes during metastatic progression of lung cancer remains elusive. Here, we functionally validate a previously described post-transcriptional pathway by which TGF-β modulates expression of interleukin-like EMT inducer (ILEI), and EMT itself. We show that poly r(C)-binding protein 1 (PCBP1) binds ILEI transcript and repress its translation. TGF-β activation leads to phosphorylation at serine-43 of PCBP1 by protein kinase Bβ/Akt2, inducing its release from the ILEI transcript and translational activation. Modulation of hnRNP E1 expression modification altered TGF-β-mediated reversal of translational silencing of ILEI transcripts and EMT. Furthermore, ILEI could induce, as well as maintain, CD24lowCD44high subpopulation in A549 cells treated with TGF-β, which might explain its capability to induce metastatic progression. These results thus validate the existence of an evolutionary conserved TGF-β-inducible post-transcriptional regulon that controls EMT and subsequent metastatic progression of lung cancer.