Expression and intracellular localization of duck enteritis virus pUL38 protein

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• 作者：Jun Xiang (1)
  Guangpeng Ma (2)
  Shunchuan Zhang (1)
  Anchun Cheng (1) (3) (4)
  Mingshu Wang (1) (3)
  Dekang Zhu (1) (3)
  Renyong Jia (3)
  Qihui Luo (3)
  Zhengli Chen (3)
  Xiaoyue Chen (1) (3) (4)
  
• 刊名：Virology Journal
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：7
• 期：1
• 全文大小：1483KB
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• 作者单位：Jun Xiang (1)
  Guangpeng Ma (2)
  Shunchuan Zhang (1)
  Anchun Cheng (1) (3) (4)
  Mingshu Wang (1) (3)
  Dekang Zhu (1) (3)
  Renyong Jia (3)
  Qihui Luo (3)
  Zhengli Chen (3)
  Xiaoyue Chen (1) (3) (4)
  
  1. Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan, Sichuan, 625014, China
  2. China Rural Technology Development Center, Beijing, 100045, China
  3. Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Yaan, Sichuan, 625014, China
  4. Epizootic Diseases Institute of Sichuan Agricultural University, Ya'an, Sichuan, 625014, China
  
• ISSN：1743-422X

文摘

Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts (DEFs). pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward, initially showing a diffuse distribution throughout the cytoplasm, and later in the nucleus. Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38.