The Effects of Ce on the Proliferation, Osteogenic Differentiation and Mineralization Function of MC3T3-E1 Cells In Vitro
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- 作者：Dandan Liu (1)
Jinchao Zhang (1)
Yaping Li (1)
Shuxiang Wang (1)
MengSu Yang (2) - 关键词：Cerium ; Proliferation ; Osteogenic differentiation ; Mineralization
- 刊名：Biological Trace Element Research
- 出版年：2012
- 出版时间：November 2012
- 年：2012
- 卷：149
- 期：2
- 页码：291-297
- 全文大小：387KB
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Jinchao Zhang (1)
Yaping Li (1)
Shuxiang Wang (1)
MengSu Yang (2)
1. College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People’s Republic of China
2. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, People’s Republic of China
文摘
The effects of Ce on the proliferation, osteogenic differentiation and mineralization function of a murine preosteoblast cell line MC3T3-E1 in vitro were investigated at cell and molecular levels. The results showed that Ce promoted the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells at concentrations of 0.0001, 0.001, 0.01, 0.1 and 1?μM, but turned to inhibit the proliferation, osteogenic differentiation and mineralization function at concentrations of 10, 100 and 1000?μM. Ce displayed the up-regulation of Runx2, BMP2, ALP, BSP, Col I and OCN genes at concentrations of 0.0001 and 0.1?μM; these genes were down-regulated in the MC3T3-E1 cells treated with 1000?μM Ce. The expression of BMP2, Runx2 and OCN proteins was promoted by Ce at concentrations of 0.0001 and 0.1?μM, but these proteins were down-regulated after 1000?μM Ce treatment. The results suggest that Ce likely up-regulates or down-regulates the expression of Runx2, which subsequently up- or down-regulates OB marker genes Col I and BMP2 at early stages and ALP and OCN at later stages of differentiation, thus causing to promote or inhibit the proliferation, osteogenic differentiation and mineralization function of MC3T3-E1 cells.