Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection

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• 作者：Murilo S. Alves ; Zamira G. Soares ; Pedro M. P. Vidigal&#8230
• 关键词：Biotic stress ; Signal transduction ; Plant defense ; Asian soybean rust ; Transcriptional control
• 刊名：Functional & Integrative Genomics
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：15
• 期：6
• 页码：685-696
• 全文大小：787 KB
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• 作者单位：Murilo S. Alves (1)
  Zamira G. Soares (1)
  Pedro M. P. Vidigal (2)
  Everaldo G. Barros (3)
  Adriana M. P. Poddanosqui (4)
  Luciano N. Aoyagi (4)
  Ricardo V. Abdelnoor (4)
  Francismar C. Marcelino-Guimar茫es (4)
  Luciano G. Fietto (1)
  
  1. Departamento de Bioqu铆mica e Biologia Molecular, Universidade Federal de Vi莽osa, Vi莽osa, Minas Gerais, 36570-000, Brazil
  2. N煤cleo de An谩lise de Biomol茅culas, NuBioMol, Universidade Federal de Vi莽osa, Vi莽osa, Minas Gerais, 36570-000, Brazil
  3. Universidade Cat贸lica de Bras铆lia, 70790-160, Bras铆lia, Distrito Federal, Brazil
  4. Embrapa Soja, Londrina, Paran谩, 86001-970, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Genetics and Genomics
  Microbial Genetics and Genomics
  Biochemistry
  Bioinformatics
  Animal Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-7948

文摘

Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of most important diseases in the soybean (Glycine max (L.) Merr.) agribusiness. The identification and characterization of genes related to plant defense responses to fungal infection are essential to develop ASR-resistant plants. In this work, we describe four soybean genes, GmbZIP62, GmbZIP105, GmbZIPE1, and GmbZIPE2, which encode transcription factors containing a basic leucine zipper (bZIP) domain from two divergent classes, and that are responsive to P. pachyrhizi infection. Molecular phylogenetic analyses demonstrated that these genes encode proteins similar to bZIP factors responsive to pathogens. Yeast transactivation assays showed that only GmbZIP62 has strong transactivation activity in yeast. In addition, three of the bZIP transcription factors analyzed were also differentially expressed by plant defense hormones, and all were differentially expressed by fungal attack, indicating that these proteins might participate in response to ASR infection. The results suggested that these bZIP proteins are part of the plant defense response to P. pachyrhizi infection, by regulating the gene expression related to ASR infection responses. These bZIP genes are potential targets to obtain new soybean genotypes resistant to ASR. Keywords Biotic stress Signal transduction Plant defense Asian soybean rust Transcriptional control