Drought stress in Pinus taeda L. induces coordinated transcript accumulation of genes involved in the homogentisate pathway

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• 作者：Océane Frelin ; Christopher Dervinis ; Jill L. Wegrzyn…
• 关键词：Pinus taeda ; Water availability ; Drought response ; Homogentisate pathway ; Phenylalanine ; Phenylalanine hydroxylase ; PtPheH ; Tyrosine
• 刊名：Tree Genetics & Genomes
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：13
• 期：1
• 全文大小：
• 刊物主题：Forestry; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Tree Biology; Biotechnology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1614-2950
• 卷排序：13

文摘

Phenylalanine is a central amino acid in plants and is the precursor of many key secondary metabolites such as lignin, phenylpropanoids, and flavonoids. Phenylalanine hydroxylase (PheH, EC 1.14.16.1) is not detected by sequence similarity in angiosperms, but it is present in gymnosperms and mosses where it is known to hydroxylate phenylalanine to produce tyrosine in vitro. However, its physiological role in gymnosperms is unclear. This study aimed to clarify the role of the gene encoding PheH in Pinus taeda (PtPheH), an economically and ecologically important tree native to the southern USA. The PtPheH gene is present as a single copy and consists of five exons and four introns. Comparison of two PtPheH alleles in P. taeda revealed a mobile element specific to the genus Pinus that was upstream of the transcriptional start site. PtPheH transcript abundance increased after introduction of exogenous phenylalanine and during water limitation. Similar highly and statistically significant shifts in transcript abundance were found for the genes involved in tyrosine synthesis and in the homogentisate pathway, but not for genes of phenylpropanoid metabolism. The coordinated accumulation of transcripts implies that under conditions of limited water availability, PtPheH may reroute phenylalanine away from lignin synthesis and into the degradative homogentisate pathway. Because PtPheH has homologs in pine, moss, and animals, it is presumably an ancient gene that has been lost in the angiosperm lineage of plants.