A rapid field test for the measurement of bovine serum immunoglobulin G using attenuated total reflectance infrared spectroscopy

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• 作者：Ibrahim Elsohaby ; Siyuan Hou ; J. Trenton McClure…
• 关键词：Bovine ; Failure of transfer of passive immunity ; Attenuated total reflectance infrared spectroscopy ; Immunoglobulin G
• 刊名：BMC Veterinary Research
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：11
• 期：1
• 全文大小：1,304 KB
• 刊物主题：Veterinary Medicine; Zoology; Transgenics;
• 出版者：BioMed Central
• ISSN：1746-6148

文摘

Background Following the recent development of a new approach to quantitative analysis of IgG concentrations in bovine serum using transmission infrared spectroscopy, the potential to measure IgG levels using technology and a device better designed for field use was investigated. A method using attenuated total reflectance infrared (ATR) spectroscopy in combination with partial least squares (PLS) regression was developed to measure bovine serum IgG concentrations. ATR spectroscopy has a distinct ease-of-use advantage that may open the door to routine point-of-care testing. Serum samples were collected from calves and adult cows, tested by a reference RID method, and ATR spectra acquired. The spectra were linked to the RID-IgG concentrations and then randomly split into two sets: calibration and prediction. The calibration set was used to build a calibration model, while the prediction set was used to assess the predictive performance and accuracy of the final model. The procedure was repeated for various spectral data preprocessing approaches.