Two types of syringomycin E channels in sphingomyelin-containing bilayers

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• 作者：Svetlana S. Efimova ; Anastasiia A. Zakharova…
• 关键词：Syringomycin E ; Planar lipid membranes ; Single ion channels ; Sphingolipids ; Lipid phase separation ; Membrane dipole potential
• 刊名：European Biophysics Journal
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：45
• 期：1
• 页码：91-98
• 全文大小：587 KB
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• 作者单位：Svetlana S. Efimova (1)
  Anastasiia A. Zakharova (1)
  Ludmila V. Schagina (1)
  Olga S. Ostroumova (1)
  
  1. Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky Ave. 4, St. Petersburg, Russia, 194064
  
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Biophysics and Biomedical Physics
  Cell Biology
  Biochemistry
  Plant Physiology
  Animal Physiology
  Neurobiology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1017

文摘

The influence of dipole modifiers on the characteristics of single syringomycin E (SRE) channels in bilayers comprising DOPS, DOPE, sphingolipids (sphingomyelin, N-stearoyl-phytosphingosine or N-stearoyl-sphinganine) and sterols (cholesterol or ergosterol) was studied. The effects of dipole modifiers on SRE channel amplitudes were dependent upon the sphingolipid type and were not affected by the membrane sterol content. A decrease in the dipole potential of phytosphingosine- and sphinganine-containing bilayers, which was induced by the adsorption of phloretin, led to a reduction in conductance; however, an increase in this potential, which occurred upon the addition of RH 421, led to an enhancement in the conductance of SRE channels. Two channel populations, one of which is sensitive while the other is insensitive to modifiers, were found in sphingomyelin-containing bilayers. This indicates that SRE channels are distributed in lipid domains with different dipole potentials.